ArticleWound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
A novel immune competent murine hypertrophic scar contracture model: a tool to elucidate disease mechanism and develop new therapies.
Article in Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
What it found
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Mesenchymal stem cell therapy in hypertrophic and keloid scars.Cell and tissue research · 2021Pooled it
- Chirality-Encoded Biomaterials Regulate Local and Systemic Immune Responses in Transplantation.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Next-generation skin wound healing related disease models with integration of immune cells.Protein & cell · 2026Review
- Piceatannol Promotes Burn Wound Healing by Coordinately Modulating Inflammation-Oxidative Stress Crosstalk, Angiogenesis, and Fibrotic Remodeling.Biomolecules · 2026Article
- Amino Acid-Based Protein-Mimic Hydrogel Incorporating Pro-Regenerative Lipid Mediator and Microvascular Fragments Promotes the Healing of Deep Burn Wounds.International journal of molecular sciences · 2024Article
- A Novel Xenograft Model Demonstrates Human Fibroblast Behavior During Skin Wound Repair and Fibrosis.Advances in wound care · 2022Article
- A Systematic Review Comparing Animal and Human Scarring Models.Frontiers in surgery · 2022Review
- A Review of the Evidence for and against a Role for Mast Cells in Cutaneous Scarring and Fibrosis.International journal of molecular sciences · 2020Review
- Inflammation as an orchestrator of cutaneous scar formation: a review of the literature.Plastic and aesthetic research · 2020Article
- Mast Cells in Skin Scarring: A Review of Animal and Human Research.Frontiers in immunology · 2020Article
- Accelerate Healing of Severe Burn Wounds by Mouse Bone Marrow Mesenchymal Stem Cell-Seeded Biodegradable Hydrogel Scaffold Synthesized from Arginine-Based Poly(ester amide) and Chitosan.Stem cells and development · 2018Article
- Article
- An immune-competent rat split thickness skin graft model: useful tools to develop new therapies to improve skin graft survival.American journal of translational research · 2018Article
- Characterization of the Capsule Surrounding Smooth and Textured Tissue Expanders and Correlation with Contracture.Plastic and reconstructive surgery. Global open · 2017Article
- Pre-vascularization Enhances Therapeutic Effects of Human Mesenchymal Stem Cell Sheets in Full Thickness Skin Wound Repair.Theranostics · 2017Article
- Shikonin reduces TGF-β1-induced collagen production and contraction in hypertrophic scar-derived human skin fibroblasts.International journal of molecular medicine · 2015Article
- Prevention of excessive scar formation using nanofibrous meshes made of biodegradable elastomer poly(3-hydroxybutyrate-Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
13 authors at 1 institution in 1 country.
Funding
Abstract
Hypertrophic scar (HSc) contraction following burn injury causes contractures. Contractures are painful and disfiguring. Current therapies are marginally effective. To study pathogenesis and develop new therapies, a murine model is needed. We have created a validated immune-competent murine HSc model. A third-degree burn was created on dorsum of C57BL/6 mice. Three days postburn, tissue was excised and grafted with ear skin. Graft contraction was analyzed and tissue harvested on different time points. Outcomes were compared with human condition to validate the model. To confirm graft survival, green fluorescent protein (GFP) mice were used, and histologic analysis was performed to differentiate between ear and back skin. Role of panniculus carnosus in contraction was analyzed. Cellularity was assessed with 4',6-diamidino-2-phenylindole. Collagen maturation was assessed with Picro-sirius red. Mast cells were stained with Toluidine blue. Macrophages were detected with F4/80 immune. Vascularity was assessed with CD31 immune. RNA for contractile proteins was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Elastic moduli of skin and scar tissue were analyzed using a microstrain analyzer. Grafts contracted to ∼45% of their original size by day 14 and maintained their size. Grafting of GFP mouse skin onto wild-type mice, and analysis of dermal thickness and hair follicle density, confirmed graft survival. Interestingly, hair follicles disappeared after grafting and regenerated in ear skin configuration by day 30. Radiological analysis revealed that panniculus carnosus doesn't contribute to contraction. Microscopic analyses showed that grafts show increase in cellularity. Granulation tissue formed after day 3. Collagen analysis revealed increases in collagen maturation over time. CD31 stain revealed increased vascularity. Macrophages and mast cells were increased. qRT-PCR showed up-regulation of transforming growth factor beta, alpha smooth muscle actin, and rho-associated protein kinase 2 in HSc. Tensile testing revealed that human skin and scar tissues are tougher than mouse skin and scar tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.