ArticlePlant cell reports2015
Development and optimization of agroinfiltration for soybean.
Article in Plant cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- Agrobacterium-mediated genetic transformation of chia (Salvia hispanica L.), a rich source of omega-3 fatty acid.Transgenic research · 2025Article
- Agroinfiltration-mediated transient assay for rapid evaluation of constructs in pigeonpea.Biotechnology notes (Amsterdam, Netherlands) · 2025Article
- Refinement and Enhancement ofGenes · 2024Article
- Robust soybean leaf agroinfiltration.Plant cell reports · 2024Article
- Comparative Evaluation of Transient Protein Expression Efficiency in Tissues across Soybean Varieties Using the Tsukuba System.Plants (Basel, Switzerland) · 2024Article
- The association between Dioscorea sansibarensis and Orrella dioscoreae as a model for hereditary leaf symbiosis.PloS one · 2024Article
- Multiple indeterminate domain (IDD)-DELLA1 complexes participate in gibberellin feedback regulation in peach.Plant molecular biology · 2022Article
- Article
- Progress in Soybean Genetic Transformation Over the Last Decade.Frontiers in plant science · 2022Review
- An Easy and Rapid Transformation Protocol for Transient Expression in Cotton Fiber.Frontiers in plant science · 2022Article
- Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants.International journal of molecular sciences · 2021Review
- Leaf infiltration in plant science: old method, new possibilities.Plant methods · 2021Review
- Agroinfiltration for transient gene expression and characterisation of fungal pathogen effectors in cool-season grain legume hosts.Plant cell reports · 2021Article
- A New Plant Expression System for Producing Pharmaceutical Proteins.Molecular biotechnology · 2020Article
- Metabolic Engineering Strategies of Industrial Hemp (Frontiers in plant science · 2020Review
- Development of Agrobacterium-mediated transient expression system in Caragana intermedia and characterization of CiDREB1C in stress response.BMC plant biology · 2019Article
- Screening of a multi-virus resistant RNAi construct in cowpea through transient vacuum infiltration method.Virusdisease · 2019Article
- Agroinfiltration-based efficient transient protein expression in leguminous plants.Plant biotechnology (Tokyo, Japan) · 2019Article
- Article
- In silico and in vivo analyses of the mutated human tissue plasminogen activator (mtPA) and the antithetical effects of P19 silencing suppressor on its expression in two Nicotiana species.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageAgroinfiltration is an efficient method to study transgene expression in plant tissue. In this study, sonication followed by vacuum infiltration is shown to increase agroinfiltration-mediated GUS expression in soybean. Agroinfiltration, a valuable tool for rapid analysis of gene function, has been used extensively on leaf tissue of Nicotiana benthamiana and several other plant species. However, the application of this approach for gene functionality studies in soybean has been largely unsuccessful. Improvements in agroinfiltration of many plants have been achieved through a variety of approaches to allow better delivery, penetration and infection of Agrobacterium to interior leaf tissues. In this work, an agroinfiltration approach was developed for transient expression in soybean utilizing sonication followed by vacuum infiltration of intact seedlings. The optimal infiltration buffer, sonication time, and vacuum conditions for agroinfiltration of soybean were evaluated by monitoring expression of an introduced β-glucuronidase (GUS) reporter gene. The developed method included the use of an infiltration buffer (10 mM 2-(N-morpholino)ethanesulfonic acid sodium salt, 10 mM MgCl2, 100 µM acetosyringone) supplemented with the reducing agent dithiothreitol, with 30 s sonication followed by vacuum infiltration. These techniques were further applied to evaluate five different Agrobacterium strains and six different plant genetic backgrounds. Among the Agrobacterium strains examined, J2 produced the highest levels of GUS activity and 'Peking' was the most responsive genotype.
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