ArticleJournal of cancer research and clinical oncology2015
Isolation and characterization of circulating tumor cells from human gastric cancer patients.
Article in Journal of cancer research and clinical oncology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 2 of them syntheses that pooled it.
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Who cites it
15 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Investigating the role of circulating tumor cells in gastric cancer: a comprehensive systematic review and meta-analysis.Clinical and experimental medicine · 2024Pooled it
- Prognostic Role of the Circulating Tumor Cells Detected by Cytological Methods in Gastric Cancer: A Meta-Analysis.BioMed research international · 2016Pooled it
- Tuftsin-tailored fusion protein inhibits the growth of circulating gastric tumor cells associated with macrophage phagocytosis.Biochemistry and biophysics reports · 2023Article
- Review
- Ascorbic Acid Inhibits Liver Cancer Growth and MetastasisFrontiers in pharmacology · 2021Article
- Review
- CD44Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association · 2019Article
- Clinical Application of Circulating Tumor Cells in Gastric Cancer.Gut and liver · 2019Review
- Differential effects of α-catenin on the invasion and radiochemosensitivity of human colorectal cancer cells.International journal of oncology · 2018Article
- Liquid biopsy provides new insights into gastric cancer.Oncotarget · 2018Review
- Review
- Clinical significance of circulating tumor cells in blood from patients with gastric cancer.Annals of gastroenterological surgery · 2017Review
- Circulating tumor cells versus circulating tumor DNA in lung cancer-which one will win?Translational lung cancer research · 2016Review
- Clinical significance of pancreatic circulating tumor cells using combined negative enrichment and immunostaining-fluorescence in situ hybridization.Journal of experimental & clinical cancer research : CR · 2016Article
- Circulating Tumor Cells, DNA, and mRNA: Potential for Clinical Utility in Patients With Melanoma.The oncologist · 2016Review
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeCirculating tumor cells (CTCs) have been proved to be responsible for tumor metastasis and resistant to anticancer therapies. This study aims to isolate and characterize circulating tumor cells from human gastric cancer patients, and investigate characteristic differences between gastric CTCs and gastric cancer cell lines.
methodsWe analyzed 31 cases of gastric cancer patients using anti-CD45 antibody-conjugated magnetic microbeads negative separation, combined with fluorescence activated cell sorter CD44 positive screening. Abilities of tumor formation, metastasis, invasion, migration, irradiation and drug sensitivity of CTCs and gastric cancer cell lines were detected and compared.
resultsOf all the 31 patients, CD44(+)/CD45(-)CTCs were isolated in 14 patients, of which 3 cases were stage IIA, 2 cases stage IIB, 2 cases stage IIIC and 7 cases stage IV. The malignant behavior was demonstrated by both clonogenetic assay and tumor xenograft in nude mice. Compared with human gastric cancer cell lines, the migration and invasion abilities of CTCs increased to 3.21-12.6-fold and 2.3-6.7-fold, respectively (all p values <0.05). In addition, the metastatic potential of CTCs is much higher in vivo than that of the control. Furthermore, CTCs were found to be relatively sensitive to FU, cisplatin and paclitaxel, but relatively resistant to irradiation, oxaliplatin, cetuximab and trastuzumab.
conclusionsCD44(+)/CD45(-) gastric CTCs were isolated and found to exhibit stronger malignant behavior when compared with human gastric cancer cell lines. Furthermore, CTCs cultured in vitro have potential implications in drug sensitivity screening for the future anticancer treatments.
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