ArticleApplied and environmental microbiology2015
An in planta-expressed polyketide synthase produces (R)-mellein in the wheat pathogen Parastagonospora nodorum.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 62 citations in OpenAlex.
- Genetic basis of Pseudonectria foliicola infection in humans and research on DKP-type natural products.BMC genomics · 2026Article
- Diversity and Distribution of Non-Reducing Polyketide Synthases (NR-PKSs) in Ascomycota (Fungi).Journal of fungi (Basel, Switzerland) · 2025Article
- Pathogenic Roles of Polyketide Synthase CLPKS18 and (Journal of fungi (Basel, Switzerland) · 2025Article
- Discovery ofJournal of fungi (Basel, Switzerland) · 2024Article
- Genome Sequencing and Analysis Reveal Potential High-Valued Metabolites Synthesized byJournal of fungi (Basel, Switzerland) · 2023Article
- Phytotoxic Metabolites Produced by Fungi Involved in Grapevine Trunk Diseases: Progress, Challenges, and Opportunities.Plants (Basel, Switzerland) · 2022Review
- Diversity ofJournal of fungi (Basel, Switzerland) · 2022Article
- The Interpretation of the Role of a Polyketide SynthaseFrontiers in microbiology · 2022Article
- Synthaser: a CD-Search enabled Python toolkit for analysing domain architecture of fungal secondary metabolite megasynth(et)ases.Fungal biology and biotechnology · 2021Article
- Saccharomyces cerevisiae as host for the recombinant production of polyketides and nonribosomal peptides.Microbial cell factories · 2021Review
- Secondary metabolite biosynthetic diversity in the fungal familyStudies in mycology · 2021Article
- Biosynthesis of 6-Hydroxymellein Requires a Collaborating Polyketide Synthase-like Enzyme.Angewandte Chemie (International ed. in English) · 2021Article
- Reference genome assembly for Australian Ascochyta lentis isolate Al4.G3 (Bethesda, Md.) · 2021Article
- Reference Genome Assembly for AustralianG3 (Bethesda, Md.) · 2020Article
- Melleins-Intriguing Natural Compounds.Biomolecules · 2020Review
- Volatile Molecules Secreted by the Wheat PathogenFrontiers in microbiology · 2020Article
- Genetic platforms for heterologous expression of microbial natural products.Natural product reports · 2019Review
- Acquisition and Loss of Secondary Metabolites Shaped the Evolutionary Path of Three Emerging Phytopathogens of Wheat.Genome biology and evolution · 2019Article
- Article
- Chemical Diversity and Biological Activities ofJournal of fungi (Basel, Switzerland) · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Parastagonospora nodorum is a pathogen of wheat that affects yields globally. Previous transcriptional analysis identified a partially reducing polyketide synthase (PR-PKS) gene, SNOG_00477 (SN477), in P. nodorum that is highly upregulated during infection of wheat leaves. Disruption of the corresponding SN477 gene resulted in the loss of production of two compounds, which we identified as (R)-mellein and (R)-O-methylmellein. Using a Saccharomyces cerevisiae yeast heterologous expression system, we successfully demonstrated that SN477 is the only enzyme required for the production of (R)-mellein. This is the first identification of a fungal PKS that is responsible for the synthesis of (R)-mellein. The P. nodorum ΔSN477 mutant did not show any significant difference from the wild-type strain in its virulence against wheat. However, (R)-mellein at 200 μg/ml inhibited the germination of wheat (Triticum aestivum) and barrel medic (Medicago truncatula) seeds. Comparative sequence analysis identified the presence of mellein synthase (MLNS) homologues in several Dothideomycetes and two sodariomycete genera. Phylogenetic analysis suggests that the MLNSs in fungi and bacteria evolved convergently from fungal and bacterial 6-methylsalicylic acid synthases.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.