ArticleApplied and environmental microbiology2015
Distribution of human-specific bacteroidales and fecal indicator bacteria in an urban watershed impacted by sewage pollution, determined using RNA- and DNA-based quantitative PCR assays.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 54 citations in OpenAlex.
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- Highlighting the promise of qPCR-based environmental monitoring: response of the ribosomal RNA:DNA ratio of calanoid copepods to toxic cyanobacteria.Ecotoxicology (London, England) · 2021Article
- Distribution and Antibiotic Resistance Profiles ofGeoHealth · 2021Article
- The Use of Ribosomal RNA as a Microbial Source Tracking Target Highlights the Assay Host-Specificity Requirement in Water Quality Assessments.Frontiers in microbiology · 2021Article
- Reaching a Large Urban Undergraduate Population through Microbial Ecology Course-Based Research Experiences.Journal of microbiology & biology education · 2020Article
- Two Drinking Water Outbreaks Caused by Wastewater Intrusion Including Sapovirus in Finland.International journal of environmental research and public health · 2019Article
- Evaluating putative ecological drivers of microcystin spatiotemporal dynamics using metabarcoding and environmental data.Harmful algae · 2019Article
- Urbanization Impacts the Physicochemical Characteristics and Abundance of Fecal Markers and Bacterial Pathogens in Surface Water.International journal of environmental research and public health · 2019Article
- Removal of fecal indicator bacteria and antibiotic resistant genes in constructed wetlands.Environmental science and pollution research international · 2019Article
- Quantification of Microbial Source Tracking and Pathogenic Bacterial Markers in Water and Sediments of Tiaoxi River (Taihu Watershed).Frontiers in microbiology · 2019Article
- Multiscale investigation of a symbiotic microalgal-integrated fixed film activated sludge (MAIFAS) process for nutrient removal and photo-oxygenation.Bioresource technology · 2018Article
- Categorical performance characteristics of method ISO 7899-2 and indicator value of intestinal enterococci for bathing water quality monitoring.Journal of water and health · 2018Article
- Analysis of human mitochondrial DNA sequences from fecally polluted environmental waters as a tool to study population diversity.AIMS environmental science · 2017Article
- Naphthoquinone glycosides for bioelectroanalytical enumeration of the faecal indicator Escherichia coli.Microbial biotechnology · 2016Article
- Use of functional gene expression and respirometry to study wastewater nitrification activity after exposure to low doses of copper.Environmental science and pollution research international · 2016Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The identification of fecal pollution sources is commonly carried out using DNA-based methods. However, there is evidence that DNA can be associated with dead cells or present as "naked DNA" in the environment. Furthermore, it has been shown that rRNA-targeted reverse transcription-quantitative PCR (RT-qPCR) assays can be more sensitive than rRNA gene-based qPCR assays since metabolically active cells usually contain higher numbers of ribosomes than quiescent cells. To this end, we compared the detection frequency of host-specific markers and fecal bacteria using RNA-based RT-qPCR and DNA-based qPCR methods for water samples collected in sites impacted by combined sewer overflows. As a group, fecal bacteria were more frequently detected in most sites using RNA-based methods. Specifically, 8, 87, and 85% of the samples positive for general enterococci, Enterococcus faecalis, and Enterococcus faecium markers, respectively, were detected using RT-qPCR, but not with the qPCR assay counterpart. On average, two human-specific Bacteroidales markers were not detected when using DNA in 12% of the samples, while they were positive for all samples when using RNA (cDNA) as the template. Moreover, signal intensity was up to three orders of magnitude higher in RT-qPCR assays than in qPCR assays. The human-specific Bacteroidales markers exhibited moderate correlation with conventional fecal indicators using RT-qPCR results, suggesting the persistence of nonhuman sources of fecal pollution or the presence of false-positive signals. In general, the results from this study suggest that RNA-based assays can increase the detection sensitivity of fecal bacteria in urban watersheds impacted with human fecal sources.
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