ArticleToxicological sciences : an official journal of the Society of Toxicology2015
Formation of a protein corona on silver nanoparticles mediates cellular toxicity via scavenger receptors.
Article in Toxicological sciences : an official journal of the Society of Toxicology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed, 157 citations in OpenAlex.
- Mapping a sustainable approach with whey-biosynthesized silver nanocomposites versus chemically synthesized silver nanoparticles in a chronic hippocampal toxicity rat model.Scientific reports · 2026Article
- Impact of an Artificial Albumin Corona on Surface Charge-Driven Nano-Bio Interactions and Cytotoxicity of Silver Nanoparticles.ACS omega · 2026Article
- The effect of protein corona formation on the nano-bio interaction of the Au/NdDiscover nano · 2026Article
- Synthesis of Metal and Metal Oxide Nanoparticles by Flame Spray Pyrolysis and Safety Assessment.Toxics · 2026Article
- Sulfidation Affects Macrophage Uptake of Metal-Based Nanomaterials by Dictating Protein Corona Compositions and Aggregation States Collectively.Environment & health (Washington, D.C.) · 2025Article
- Silver integrated hybrids and nanocomposites for next-generation biomedicine: Beyond antimicrobial coatings toward smart sense-response-heal platforms.Materials today. Bio · 2025Review
- Article
- Research progress on mRNA drug delivery strategies in the nervous system.Nanomedicine (London, England) · 2025Review
- Mechanistic Modeling of Size and Surface Charge Changes of Gold and Silver Nanoparticles during In Vitro Nanoparticle Protein Corona Formation.ACS pharmacology & translational science · 2025Article
- Antibacterial and Anti-Adherence Efficacy of Silver Nanoparticles Against Endodontic Biofilms: An In Vitro and Ex Vivo Study.Pharmaceutics · 2025Article
- Do We Know Enough About the Safety Profile of Silver Nanoparticles in Oncology? A Focus on Novel Methods and Approaches.International journal of molecular sciences · 2025Review
- Silver Nanoparticle-Mediated Antiviral Efficacy against Enveloped Viruses: A Comprehensive Review.Global challenges (Hoboken, NJ) · 2025Review
- Silver Nanoparticles Decorated UiO-66-NHPharmaceutics · 2025Article
- Humanising nanotoxicology: replacement of animal-derived products in the application of integrated approaches to testing and assessment of nanomaterial inhalation hazard.Frontiers in bioengineering and biotechnology · 2025Article
- The in vitro gastrointestinal digestion-associated protein corona of polystyrene nano- and microplastics increases their uptake by human THP-1-derived macrophages.Particle and fibre toxicology · 2024Article
- Scavenger receptor BI attenuates oxidized phospholipid-induced pulmonary inflammation.Toxicology and applied pharmacology · 2023Article
- Silver Nanoparticle Surface Chemistry Determines Interactions with Human Serum Albumin and Cytotoxic Responses in Human Liver Cells.ACS omega · 2023Article
- Serum protein coating enhances the antisepsis efficacy of silver nanoparticles against multidrug-resistantFrontiers in microbiology · 2023Article
- Silver nanoparticle interactions with glycated and non-glycated human serum albumin mediate toxicity.Frontiers in toxicology · 2023Article
- Overview of mechanism and consequences of endothelial leakiness caused by metal and polymeric nanoparticles.Beilstein journal of nanotechnology · 2023Review
14 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Addition of a protein corona (PC) or protein adsorption layer on the surface of nanomaterials following their introduction into physiological environments may modify their activity, bio-distribution, cellular uptake, clearance, and toxicity. We hypothesize that silver nanoparticles (AgNPs) will associate with proteins common to human serum and cell culture media forming a PC that will impact cell activation and cytotoxicity. Furthermore, the role of scavenger receptor BI (SR-BI) in mediating this toxicity was evaluated. Citrate-suspended 20 nm AgNPs were incubated with human serum albumin (HSA), bovine serum albumin (BSA), high-density lipoprotein (HDL), or water (control) to form a PC. AgNPs associated with each protein (HSA, BSA, and HDL) forming PCs as assessed by electron microscopy, hyperspectral analysis, ζ-potential, and hydrodynamic size. Addition of the PC decreased uptake of AgNPs by rat lung epithelial and rat aortic endothelial cells. Hyperspectral analysis demonstrated a loss of the AgNP PC following internalization. Cells demonstrated concentration-dependent cytotoxicity following exposure to AgNPs with or without PCs (0, 6.25, 12.5, 25 or 50 μg/ml). All PC-coated AgNPs were found to activate cells by inducing IL-6 mRNA expression. A small molecule SR-BI inhibitor was utilized to determine the role of SR-BI in the observed effects. Pretreatment with the SR-BI inhibitor decreased internalization of AgNPs with or without PCs, and reduced both cytotoxicity and IL-6 mRNA expression. This study characterizes the formation of a PC on AgNPs and demonstrates its influence on cytotoxicity and cell activation through a cell surface receptor.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.