ArticleBMC plant biology2014
Identification of QTLs affecting scopolin and scopoletin biosynthesis in Arabidopsis thaliana.
Article in BMC plant biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 57 citations in OpenAlex.
- CYP98A monooxygenases: a key enzyme family in plant phenolic compound biosynthesis.Horticulture research · 2025Article
- Development of a Matrix-Assisted Laser Desorption Ionization High Resolution Mass Spectrometry Method for the Quantification of Camalexin and Scopoletin in Arabidopsis thaliana.Rapid communications in mass spectrometry : RCM · 2025Article
- β-Carotene alleviates substrate inhibition caused by asymmetric cooperativity.Nature communications · 2025Article
- Integration of QTL mapping and GWAS reveals the complicated genetic architecture of chemical composition traits in tobacco leaves.Frontiers in plant science · 2025Article
- Genes to specialized metabolites: accumulation of scopoletin, umbelliferone and their glycosides in natural populations of Arabidopsis thaliana.BMC plant biology · 2024Article
- MAPK and phenylpropanoid metabolism pathways involved in regulating the resistance of upland cotton plants toFrontiers in plant science · 2024Article
- Engineered coumarin accumulation reduces mycotoxin-induced oxidative stress and disease susceptibility.Plant biotechnology journal · 2023Article
- Metabolites and Plant Hormones Related to the Resistance Response to Feeding Stimulation and Leaf Clipping Control in Chinese Pine (Current issues in molecular biology · 2023Article
- Rapid defense mechanism suppression during viral- oomycete disease complex formation.Frontiers in plant science · 2023Article
- Acute and Rapid Response ofPlants (Basel, Switzerland) · 2022Article
- cis-prenyltransferase 3 and α/β-hydrolase are new determinants of dolichol accumulation in Arabidopsis.Plant, cell & environment · 2022Article
- Transcriptomic Changes in Internode Explants of Stinging Nettle during Callogenesis.International journal of molecular sciences · 2021Article
- Interplay between Coumarin Accumulation, Iron Deficiency and Plant Resistance toInternational journal of molecular sciences · 2021Article
- Effect ofPlants (Basel, Switzerland) · 2021Article
- Identification and Quantification of Coumarins by UHPLC-MS inMolecules (Basel, Switzerland) · 2021Article
- Root-Secreted Coumarins and the Microbiota Interact to Improve Iron Nutrition in Arabidopsis.Cell host & microbe · 2020Article
- Scopoletin 8-hydroxylase: a novel enzyme involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis.Journal of experimental botany · 2018Article
- Gene Expression Profiling of Iron Deficiency Chlorosis Sensitive and Tolerant Soybean Indicates Key Roles for Phenylpropanoids under Alkalinity Stress.Frontiers in plant science · 2018Article
- SG2-Type R2R3-MYB Transcription Factor MYB15 Controls Defense-Induced Lignification and Basal Immunity in Arabidopsis.The Plant cell · 2017Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundScopoletin and its glucoside scopolin are important secondary metabolites synthesized in plants as a defense mechanism against various environmental stresses. They belong to coumarins, a class of phytochemicals with significant biological activities that is widely used in medical application and cosmetics industry. Although numerous studies showed that a variety of coumarins occurs naturally in several plant species, the details of coumarins biosynthesis and its regulation is not well understood. It was shown previously that coumarins (predominantly scopolin and scopoletin) occur in Arabidopsis thaliana (Arabidopsis) roots, but until now nothing is known about natural variation of their accumulation in this model plant. Therefore, the genetic architecture of coumarins biosynthesis in Arabidopsis has not been studied before.
resultsHere, the variation in scopolin and scopoletin content was assessed by comparing seven Arabidopsis accessions. Subsequently, a quantitative trait locus (QTL) mapping was performed with an Advanced Intercross Recombinant Inbred Lines (AI-RILs) mapping population EstC (Est-1 × Col). In order to reveal the genetic basis of both scopolin and scopoletin biosynthesis, two sets of methanol extracts were made from Arabidopsis roots and one set was additionally subjected to enzymatic hydrolysis prior to quantification done by high-performance liquid chromatography (HPLC). We identified one QTL for scopolin and five QTLs for scopoletin accumulation. The identified QTLs explained 13.86% and 37.60% of the observed phenotypic variation in scopolin and scopoletin content, respectively. In silico analysis of genes located in the associated QTL intervals identified a number of possible candidate genes involved in coumarins biosynthesis.
conclusionsTogether, our results demonstrate for the first time that Arabidopsis is an excellent model for studying the genetic and molecular basis of natural variation in coumarins biosynthesis in plants. It additionally provides a basis for fine mapping and cloning of the genes involved in scopolin and scopoletin biosynthesis. Importantly, we have identified new loci for this biosynthetic process.
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