ArticleBiomacromolecules2014
Efficient targeting of fatty-acid modified oligonucleotides to live cell membranes through stepwise assembly.
Article in Biomacromolecules, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed.
- Photolabile oligonucleotides with topological light gradients enable spatially resolved single-cell transcriptomics and epigenomics.Nature biotechnology · 2026Article
- Developmentally inspired synthetic kidney engineering.Nature biotechnology · 2026Review
- fastdemux: Robust SNP-based demultiplexing of single-cell population genomics data.bioRxiv : the preprint server for biology · 2026Article
- Article
- Controlling Cell Interactions with DNA Directed Assembly.Advanced healthcare materials · 2024Review
- Highly parallel production of designer organoids by mosaic patterning of progenitors.Cell systems · 2024Article
- Structural DNA nanotechnology at the nexus of next-generation bio-applications: challenges and perspectives.Nanoscale advances · 2024Review
- High-Throughput Assembly of Compositionally Controlled 3D Cell Communities for Developmental Engineering.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Advancing cell surface modification in mammalian cells with synthetic molecules.Chemical science · 2023Review
- Nucleic acid amphiphiles: Synthesis, properties, and applications.Molecular therapy. Nucleic acids · 2023Review
- Polymeric biomaterial-inspired cell surface modulation for the development of novel anticancer therapeutics.Biomaterials research · 2023Review
- Independent control over cell patterning and adhesion on hydrogel substrates for tissue interface mechanobiology.iScience · 2023Article
- A reductionist approach to determine the effect of cell-cell contact on human epidermal stem cell differentiation.Acta biomaterialia · 2022Article
- Sample-multiplexing approaches for single-cell sequencing.Cellular and molecular life sciences : CMLS · 2022Review
- Detecting Intact Virus Using Exogenous Oligonucleotide Labels.Analytical chemistry · 2022Article
- Article
- DNA-Based Molecular Engineering of the Cell Membrane.Membranes · 2022Review
- Multiplexed DNA-Directed Patterning of Antibodies for Applications in Cell Subpopulation Analysis.ACS applied materials & interfaces · 2021Article
- POLYseq: A poly(β-amino ester)-based vector for multifunctional cellular barcoding.Stem cell reports · 2021Article
- Multiplexing Methods for Simultaneous Large-Scale Transcriptomic Profiling of Samples at Single-Cell Resolution.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Lipid modifications provide efficient targeting of oligonucleotides to live cell membranes in a range of applications. Targeting efficiency is a function of the rate of lipid DNA insertion into the cell surface and its persistence over time. Here we show that increasing lipid hydrophobicity increases membrane persistence, but decreases the rate of membrane insertion due to the formation of nonproductive aggregates in solution. To ameliorate this effect, we split the net hydrophobicity of the membrane anchor between two complementary oligonucleotides. When prehybridized in solution, doubly anchored molecules also aggregate due to their elevated hydrophobicity. However, when added sequentially to cells, aggregation does not occur so membrane insertion is efficient. Hybridization between the two strands locks the complexes at the cell surface by increasing net hydrophobicity, increasing their total concentration and lifetime, and dramatically improving their utility in a variety of biomedical applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.