Evidence map›Paper›PMID 25324846›Full record

ReviewFrontiers in plant science2014

Genotyping-by-sequencing (GBS), an ultimate marker-assisted selection (MAS) tool to accelerate plant breeding.

Jiangfeng He, Xiaoqing Zhao, André Laroche, Zhen-Xiang Lu, HongKui Liu, Ziqin Li

Abstract readReview
In one paragraph

Review in Frontiers in plant science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 258 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
258citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

258 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  13. The Bra1K array: an efficient and versatile 1K liquid-phase SNP chip for genetics and breeding in Chinese cabbage.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026
    Article
  14. Review
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  16. Rapid Identification of Candidate SNPs and QTLs forCurrent issues in molecular biology · 2026
    Article
  17. Article
  18. GBS-based identification ofFrontiers in plant science · 2026
    Article
  19. Article
  20. Article

198 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jiangfeng HeInner Mongolia Academy of Agriculture and Husbandry Science Hohhot, China ; Lethbridge Research Centre, Agriculture and Agri-Food Canada Lethbridge, AB, Canada.
Xiaoqing ZhaoInner Mongolia Academy of Agriculture and Husbandry Science Hohhot, China.
André LarocheLethbridge Research Centre, Agriculture and Agri-Food Canada Lethbridge, AB, Canada.
Zhen-Xiang LuLethbridge Research Centre, Agriculture and Agri-Food Canada Lethbridge, AB, Canada.
HongKui LiuInner Mongolia Academy of Agriculture and Husbandry Science Hohhot, China.
Ziqin LiInner Mongolia Academy of Agriculture and Husbandry Science Hohhot, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Marker-assisted selection (MAS) refers to the use of molecular markers to assist phenotypic selections in crop improvement. Several types of molecular markers, such as single nucleotide polymorphism (SNP), have been identified and effectively used in plant breeding. The application of next-generation sequencing (NGS) technologies has led to remarkable advances in whole genome sequencing, which provides ultra-throughput sequences to revolutionize plant genotyping and breeding. To further broaden NGS usages to large crop genomes such as maize and wheat, genotyping-by-sequencing (GBS) has been developed and applied in sequencing multiplexed samples that combine molecular marker discovery and genotyping. GBS is a novel application of NGS protocols for discovering and genotyping SNPs in crop genomes and populations. The GBS approach includes the digestion of genomic DNA with restriction enzymes followed by the ligation of barcode adapter, PCR amplification and sequencing of the amplified DNA pool on a single lane of flow cells. Bioinformatic pipelines are needed to analyze and interpret GBS datasets. As an ultimate MAS tool and a cost-effective technique, GBS has been successfully used in implementing genome-wide association study (GWAS), genomic diversity study, genetic linkage analysis, molecular marker discovery and genomic selection under a large scale of plant breeding programs.

Indexed as

genomic selection (GS)genotyping-by-sequencing (GBS)marker-assisted selection (MAS)next-generation sequencing (NGS)single nucleotide polymorphism (SNP)

Identifiers

PMID25324846
PMCPMC4179701

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.