ArticleJournal of radiation research2015
Co-visualization of DNA damage and ion traversals in live mammalian cells using a fluorescent nuclear track detector.
Article in Journal of radiation research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 33 citations in OpenAlex.
- Biosensor Cell-Fit-HDSTAR protocols · 2022Article
- Raman Research on Bleomycin-Induced DNA Strand Breaks and Repair Processes in Living Cells.International journal of molecular sciences · 2022Article
- Biosensor for deconvolution of individual cell fate in response to ion beam irradiation.Cell reports methods · 2022Article
- Medium-thickness-dependent proton dosimetry for radiobiological experiments.Scientific reports · 2019Article
- Evidence of Local Concentration of α-Particles fromJournal of nuclear medicine : official publication, Society of Nuclear Medicine · 2019Article
- Single α-particle irradiation permits real-time visualization of RNF8 accumulation at DNA damaged sites.Scientific reports · 2017Article
- Nanoscale measurements of proton tracks using fluorescent nuclear track detectors.Medical physics · 2016Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The geometric locations of ion traversals in mammalian cells constitute important information in the study of heavy ion-induced biological effect. Single ion traversal through a cellular nucleus produces complex and massive DNA damage at a nanometer level, leading to cell inactivation, mutations and transformation. We present a novel approach that uses a fluorescent nuclear track detector (FNTD) for the simultaneous detection of the geometrical images of ion traversals and DNA damage in single cells using confocal microscopy. HT1080 or HT1080-53BP1-GFP cells were cultured on the surface of a FNTD and exposed to 5.1-MeV/n neon ions. The positions of the ion traversals were obtained as fluorescent images of a FNTD. Localized DNA damage in cells was identified as fluorescent spots of γ-H2AX or 53BP1-GFP. These track images and images of damaged DNA were obtained in a short time using a confocal laser scanning microscope. The geometrical distribution of DNA damage indicated by fluorescent γ-H2AX spots in fixed cells or fluorescent 53BP1-GFP spots in living cells was found to correlate well with the distribution of the ion traversals. This method will be useful for evaluating the number of ion hits on individual cells, not only for micro-beam but also for random-beam experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.