ArticleDNA research : an international journal for rapid publication of reports on genes and genomes2015
Highly sensitive targeted methylome sequencing by post-bisulfite adaptor tagging.
Article in DNA research : an international journal for rapid publication of reports on genes and genomes, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Upregulated Ribosomal Pathway Impairs Follicle Development in a Polycystic Ovary Syndrome Mouse Model: Differential Gene Expression Analysis of Oocytes.Reproductive sciences (Thousand Oaks, Calif.) · 2023Article
- Persistent epigenetic alterations in transcription factors after a sustained virological response in hepatocellular carcinoma.JGH open : an open access journal of gastroenterology and hepatology · 2022Article
- The microRNA cluster C19MC confers differentiation potential into trophoblast lineages upon human pluripotent stem cells.Nature communications · 2022Article
- Analysis and Performance Assessment of the Whole Genome Bisulfite Sequencing Data Workflow: Currently Available Tools and a Practical Guide to Advance DNA Methylation Studies.Small methods · 2022Review
- DNA Methylation: A Promising Approach in Management of Alzheimer's Disease and Other Neurodegenerative Disorders.Biology · 2022Review
- Low Input Genome-Wide DNA Methylation Analysis with Minimal Library Amplification.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Current and Emerging Technologies for the Analysis of the Genome-Wide and Locus-Specific DNA Methylation Patterns.Advances in experimental medicine and biology · 2022Article
- Fast and memory-efficient mapping of short bisulfite sequencing reads using a two-letter alphabet.NAR genomics and bioinformatics · 2021Article
- Determining subpopulation methylation profiles from bisulfite sequencing data of heterogeneous samples using DXM.Nucleic acids research · 2021Article
- An Optimized Method for the Construction of a DNA Methylome from Small Quantities of Tissue or Purified DNA fromMolecules and cells · 2021Article
- In Response to Abiotic Stress, DNA Methylation Confers EpiGenetic Changes in Plants.Plants (Basel, Switzerland) · 2021Review
- Article
- Estimating Global Methylation and Erasure Using Low-Coverage Whole-Genome Bisulfite Sequencing (WGBS ).Methods in molecular biology (Clifton, N.J.) · 2021Article
- Extraction and high-throughput sequencing of oak heartwood DNA: Assessing the feasibility of genome-wide DNA methylation profiling.PloS one · 2021Article
- Is DNA Methylation a Ray of Sunshine in Predicting Meningioma Prognosis?Frontiers in oncology · 2020Article
- Highly efficient single-stranded DNA ligation technique improves low-input whole-genome bisulfite sequencing by post-bisulfite adaptor tagging.Nucleic acids research · 2019Article
- Mapping of histone-binding sites in histone replacement-completed spermatozoa.Nature communications · 2018Article
- "Same difference": comprehensive evaluation of four DNA methylation measurement platforms.Epigenetics & chromatin · 2018Article
- Accurate estimation of 5-methylcytosine in mammalian mitochondrial DNA.Scientific reports · 2018Article
- DNMTs and SETDB1 function as co-repressors in MAX-mediated repression of germ cell-related genes in mouse embryonic stem cells.PloS one · 2018Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The current gold standard method for methylome analysis is whole-genome bisulfite sequencing (WGBS), but its cost is substantial, especially for the purpose of multi-sample comparison of large methylomes. Shotgun bisulfite sequencing of target-enriched DNA, or targeted methylome sequencing (TMS), can be a flexible, cost-effective alternative to WGBS. However, the current TMS protocol requires a considerable amount of input DNA and hence is hardly applicable to samples of limited quantity. Here we report a method to overcome this limitation by using post-bisulfite adaptor tagging (PBAT), in which adaptor tagging is conducted after bisulfite treatment to circumvent bisulfite-induced loss of intact sequencing templates, thereby enabling TMS of a 100-fold smaller amount of input DNA with far fewer cycles of polymerase chain reaction than in the current protocol. We thus expect that the PBAT-mediated TMS will serve as an invaluable method in epigenomics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.