ArticleScientific reports2014
Production of salidroside in metabolically engineered Escherichia coli.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 95 citations in OpenAlex.
- Salidroside and exercise performance in healthy active young adults - an exploratory, randomized, double-blind, placebo-controlled study.Journal of the International Society of Sports Nutrition · 2024Trial
- Comparative analysis of precursor supply and pathway reinforcement strategies for shikimic acid biosynthesis in Pseudomonas putida.Microbial cell factories · 2026Article
- Systems metabolic engineering for hydroxytyrosol production inApplied and environmental microbiology · 2026Article
- Valorization of ligustrum species: biosynthesis, metabolic engineering, and pharmacology of bioactive compounds.Bioresources and bioprocessing · 2026Review
- Metabolic engineering ofApplied and environmental microbiology · 2025Article
- Efficient synthesis of salidroside using mined glycosyltransferase through cascade reaction.Bioprocess and biosystems engineering · 2025Article
- Production of aromatic amino acids and their derivatives by Escherichia coli and Corynebacterium glutamicum.World journal of microbiology & biotechnology · 2025Review
- Complete pathway elucidation of echinacoside in Cistanche tubulosa and de novo biosynthesis of phenylethanoid glycosides.Nature communications · 2025Article
- Expanding the application of tyrosine: engineering microbes for the production of tyrosine and its derivatives.Frontiers in bioengineering and biotechnology · 2025Review
- Integrated transcriptome and metabolome provide insight into phenolics and soluble sugar variation in the different varieties ofFrontiers in plant science · 2025Article
- Salidroside: An Overview of Its Promising Potential and Diverse Applications.Pharmaceuticals (Basel, Switzerland) · 2024Review
- Transcriptome and metabolome analysis revealed the dynamic change of bioactive compounds of Fructus Ligustri Lucidi.BMC plant biology · 2024Article
- Molecular Identification of UDP-Sugar-Dependent Glycosyltransferase and Acyltransferase Involved in the Phenylethanoid Glycoside Biosynthesis Induced by Methyl Jasmonate in Sesamum indicum L.Plant & cell physiology · 2023Article
- Complete biosynthesis of the phenylethanoid glycoside verbascoside.Plant communications · 2023Article
- Design and construction of microbial cell factories based on systems biology.Synthetic and systems biotechnology · 2023Review
- Improvement of antioxidant properties of jujube puree by biotransformation of polyphenols viaFood chemistry: X · 2022Article
- Integrating transcriptome and chemical analyses to reveal the anti-Alzheimer's disease components inFrontiers in plant science · 2022Article
- Multi-modular engineering of Saccharomyces cerevisiae for high-titre production of tyrosol and salidroside.Microbial biotechnology · 2021Article
- Variability of Major Phenyletanes and Phenylpropanoids in 16-Year-OldMolecules (Basel, Switzerland) · 2020Article
- Traditional Asian Herbs in Skin Whitening: The Current Development and Limitations.Frontiers in pharmacology · 2020Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salidroside (1) is the most important bioactive component of Rhodiola (also called as "Tibetan Ginseng"), which is a valuable medicinal herb exhibiting several adaptogenic properties. Due to the inefficiency of plant extraction and chemical synthesis, the supply of salidroside (1) is currently limited. Herein, we achieved unprecedented biosynthesis of salidroside (1) from glucose in a microorganism. First, the pyruvate decarboxylase ARO10 and endogenous alcohol dehydrogenases were recruited to convert 4-hydroxyphenylpyruvate (2), an intermediate of L-tyrosine pathway, to tyrosol (3) in Escherichia coli. Subsequently, tyrosol production was improved by overexpressing the pathway genes, and by eliminating competing pathways and feedback inhibition. Finally, by introducing Rhodiola-derived glycosyltransferase UGT73B6 into the above-mentioned recombinant strain, salidroside (1) was produced with a titer of 56.9 mg/L. Interestingly, the Rhodiola-derived glycosyltransferase, UGT73B6, also catalyzed the attachment of glucose to the phenol position of tyrosol (3) to form icariside D2 (4), which was not reported in any previous literatures.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.