Evidence map›Paper›PMID 25322934›Full record

ArticleScientific reports2014

Development of an immunologically tolerated combination of fluorescent proteins for in vivo two-photon imaging.

Selamawit Gossa, Debasis Nayak, Bernd H Zinselmeyer, Dorian B McGavern

Abstract read
In one paragraph

Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
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  5. Tracing LYVE1bioRxiv : the preprint server for biology · 2025
    Article
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  7. Article
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  14. Review
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  18. T-bet-dependent NKp46Nature immunology · 2017
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Selamawit GossaNational Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
Debasis NayakNational Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
Bernd H ZinselmeyerNational Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
Dorian B McGavernNational Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.

Funding

Pathogenesis of viral meningitisZIANS003112 · NINDS · NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE · PI MCGAVERN, DORIAN · 2009 to 2025
$33.6M
Intramural NIH HHS
6 · The paper itself

Abstract

Combinations of fluorescent proteins (FPs) are routinely used for multi-parameter in vivo imaging experiments to visualize tagged proteins or cell populations of interest. Studies involving FPs are often limited by spectral overlap, toxicity, relative quantum efficiency, and the potential for immunological rejection upon transfer into a non-tolerant recipient. Here we evaluate the immunologic visibility of several commonly used FPs by the murine immune system and identify a spectrally compatible, immunologically tolerated combination of FPs well suited for in vivo two-photon imaging.

Indexed as

Diagnostic ImagingAnimalsFluorescence Resonance Energy TransferGreen Fluorescent ProteinsImmune SystemImmune ToleranceMicePhotonsGreen Fluorescent Proteins

Identifiers

PMID25322934
PMCPMC4200419

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.