ArticleNature protocols2014
Zernike phase-contrast electron cryotomography applied to marine cyanobacteria infected with cyanophages.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 24 citations in OpenAlex.
- Three-dimensional electron ptychography of organic-inorganic hybrid nanostructures.Nature communications · 2022Article
- Cryogenic Super-Resolution Fluorescence and Electron Microscopy Correlated at the Nanoscale.Annual review of physical chemistry · 2021Review
- Setting the dynein motor in motion: New insights from electron tomography.The Journal of biological chemistry · 2019Review
- Visualizing Individual RuBisCO and Its Assembly into Carboxysomes in Marine Cyanobacteria by Cryo-Electron Tomography.Journal of molecular biology · 2018Article
- Structural variability and complexity of the giant Pithovirus sibericum particle revealed by high-voltage electron cryo-tomography and energy-filtered electron cryo-microscopy.Scientific reports · 2017Article
- Convolutional neural networks for automated annotation of cellular cryo-electron tomograms.Nature methods · 2017Article
- A new view into prokaryotic cell biology from electron cryotomography.Nature reviews. Microbiology · 2016Review
- Membrane protein structures without crystals, by single particle electron cryomicroscopy.Current opinion in structural biology · 2015Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Advances in electron cryotomography have provided new opportunities to visualize the internal 3D structures of a bacterium. An electron microscope equipped with Zernike phase-contrast optics produces images with markedly increased contrast compared with images obtained by conventional electron microscopy. Here we describe a protocol to apply Zernike phase plate technology for acquiring electron tomographic tilt series of cyanophage-infected cyanobacterial cells embedded in ice, without staining or chemical fixation. We detail the procedures for aligning and assessing phase plates for data collection, and methods for obtaining 3D structures of cyanophage assembly intermediates in the host by subtomogram alignment, classification and averaging. Acquiring three or four tomographic tilt series takes ∼12 h on a JEM2200FS electron microscope. We expect this time requirement to decrease substantially as the technique matures. The time required for annotation and subtomogram averaging varies widely depending on the project goals and data volume.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.