Evidence map›Paper›PMID 25320527›Full record

ArticleWorld journal of gastroenterology2014

Distribution of the P2X2 receptor and chemical coding in ileal enteric neurons of obese male mice (ob/ob).

Márcia Sanae Mizuno, Amanda Rabello Crisma, Primavera Borelli, Bárbara Tavares Schäfer, Mariana Póvoa Silveira, Patricia Castelucci

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in World journal of gastroenterology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.4field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Review
  5. Effects of aging on the architecture of the ileocecal junction in rats.World journal of gastrointestinal pharmacology and therapeutics · 2016
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Márcia Sanae MizunoMárcia Sanae Mizuno, Patricia Castelucci, Department of Anatomy/Biomedical Science Institute, University of São Paulo, São Paulo 05508-900, Brazil.
Amanda Rabello CrismaMárcia Sanae Mizuno, Patricia Castelucci, Department of Anatomy/Biomedical Science Institute, University of São Paulo, São Paulo 05508-900, Brazil.
Primavera BorelliMárcia Sanae Mizuno, Patricia Castelucci, Department of Anatomy/Biomedical Science Institute, University of São Paulo, São Paulo 05508-900, Brazil.
Bárbara Tavares SchäferMárcia Sanae Mizuno, Patricia Castelucci, Department of Anatomy/Biomedical Science Institute, University of São Paulo, São Paulo 05508-900, Brazil.
Mariana Póvoa SilveiraMárcia Sanae Mizuno, Patricia Castelucci, Department of Anatomy/Biomedical Science Institute, University of São Paulo, São Paulo 05508-900, Brazil.
Patricia CastelucciMárcia Sanae Mizuno, Patricia Castelucci, Department of Anatomy/Biomedical Science Institute, University of São Paulo, São Paulo 05508-900, Brazil.
Institute of Biomedical Science · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimTo investigate the colocalization, density and profile of neuronal areas of enteric neurons in the ileum of male obese mice.

methodsThe small intestinal samples of male mice in an obese group (OG) (C57BL/6J ob/ob) and a control group (CG) (+/+) were used. The tissues were analyzed using a double immunostaining technique for immunoreactivity (ir) of the P2X2 receptor, nitric oxide synthase (NOS), choline acetyl transferase (ChAT) and calretinin (Calr). Also, we investigated the density and profile of neuronal areas of the NOS-, ChAT- and Calr-ir neurons in the myenteric plexus. Myenteric neurons were labeled using an NADH-diaphorase histochemical staining method.

resultsThe analysis demonstrated that the P2X2 receptor was expressed in the cytoplasm and in the nuclear and cytoplasmic membranes only in the CG. Neuronal density values (neuron/cm(2)) decreased 31% (CG: 6579 ± 837; OG: 4556 ± 407) and 16.5% (CG: 7796 ± 528; OG: 6513 ± 610) in the NOS-ir and calretinin-ir neurons in the OG, respectively (P < 0.05). Density of ChAT-ir (CG: 6200 ± 310; OG: 8125 ± 749) neurons significantly increased 31% in the OG (P < 0.05). Neuron size studies demonstrated that NOS, ChAT, and Calr-ir neurons did not differ significantly between the CG and OG groups. The examination of NADH-diaphorase-positive myenteric neurons revealed an overall similarity between the OG and CG.

conclusionObesity may exert its effects by promoting a decrease in P2X2 receptor expression and modifications in the density of the NOS-ir, ChAT-ir and CalR-ir myenteric neurons.

Indexed as

AnimalsCalbindin 2Choline O-AcetyltransferaseDisease Models, AnimalDown-RegulationEnteric Nervous SystemIleumImmunohistochemistryMaleMice, Inbred C57BLMice, ObeseNitric Oxide SynthaseObesityReceptors, Purinergic P2X2Calb2 protein, mouseCalbindin 2Choline O-AcetyltransferaseNitric Oxide SynthaseP2rx2 protein, mouseReceptors, Purinergic P2X2Chemical codingMyenteric neuronsObesityP2X2 receptor

Identifiers

PMID25320527
PMCPMC4194573
OpenAlexW1975838868

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.