ArticlePhysiology and molecular biology of plants : an international journal of functional plant biology2014
In vitro regeneration and Agrobacterium mediated genetic transformation of Artemisia aucheri Boiss.
Article in Physiology and molecular biology of plants : an international journal of functional plant biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed.
- One-Step Regeneration of Hairy Roots to Induce High Tanshinone Plants inFrontiers in plant science · 2022Article
- Influence of different3 Biotech · 2018Article
- Production of Plant Secondary Metabolites: Examples, Tips and Suggestions for Biotechnologists.Genes · 2018Review
- In vitro regeneration and optimization of factors affecting Agrobacterium mediated transformation in Artemisia Pallens, an important medicinal plant.Physiology and molecular biology of plants : an international journal of functional plant biology · 2016Article
- Enhanced production of hyoscyamine and scopolamine from genetically transformed root culture ofIn vitro cellular & developmental biology. Plant : journal of the Tissue Culture AssociationArticle
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4 authors.
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Abstract
In the present study, we developed an efficient protocol for in vitro plant regeneration and genetically transformed root induction in medicinal plant Artemisia aucheri Boiss. Leaf explants were cultivated in MS medium supplemented by combination of plant growth regulators including α-naphthalene-acetic acid, 6-benzyl-aminopurine, indole-3-acetic acid and 2, 4-dichlorophenoxyaceticacid. The highest frequency of shoot organogenesis occurred on MS medium supplemented with 0.05 mg/l NAA plus 2 mg/l BA (96.3 %) and MS medium supplemented with 0.5 mg/l IAA plus 2 mg/l BA (88.3 %). Root induction was obtained on MS medium supplemented with 0.5 mg/l IBA. This is a simple, reliable, rapid and high efficient regeneration system for A. aucheri Boiss in short period via adventitious shoot induction approach. Also, an efficient genetically transformed root induction for A. aucheri was developed through Agrobacterium rhizogenes-mediated transformation by four bacterial strains, A4, ATCC15834, MSU440, and A13 (MAFF-02-10266). The maximum frequency of hairy root induction was obtained using MSU440 (93 %) and ATCC15834 (89 %) bacterial strains. Hairy root lines were confirmed by PCR using the rolB gene specific primers and Southern blot analysis.
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