ArticleMedical molecular morphology2015
Differentiation of mouse iPS cells into ameloblast-like cells in cultures using medium conditioned by epithelial cell rests of Malassez and gelatin-coated dishes.
Article in Medical molecular morphology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Next-Generation Strategies for Enamel Repair and Regeneration: Advances in Biomaterials and Translational Challenges.Tissue engineering and regenerative medicine · 2025Review
- Expression ofInternational journal of molecular sciences · 2025Article
- From Pluripotent Stem Cells to Organoids and Bioprinting: Recent Advances in Dental Epithelium and Ameloblast Models to Study Tooth Biology and Regeneration.Stem cell reviews and reports · 2024Review
- Mammalian dental diversity: an evolutionary template for regenerative dentistry.Frontiers in dental medicine · 2023Review
- Stage-Specific Role ofInternational journal of molecular sciences · 2021Article
- Optimization of culture conditions for the efficient differentiation of mouse-induced pluripotent stem cells into dental epithelial-like cells.In vitro cellular & developmental biology. Animal · 2020Article
- Induced Pluripotent Stem Cells in Dental and Nondental Tissue Regeneration: A Review of an Unexploited Potential.Stem cells international · 2020Review
- Tissue Engineering Approaches for Enamel, Dentin, and Pulp Regeneration: An Update.Stem cells international · 2020Review
- Case report of a molar-root incisor malformation in a patient with an autoimmune lymphoproliferative syndrome.BMC oral health · 2019Article
- Bio-implant as a novel restoration for tooth loss.Scientific reports · 2017Article
- A cancer stem cell model as the point of origin of cancer-associated fibroblasts in tumor microenvironment.Scientific reports · 2017Article
- Application of Stem Cells in Oral Disease Therapy: Progresses and Perspectives.Frontiers in physiology · 2017Review
- Induced Pluripotent Stem (iPS) Cells in Dentistry: A Review.International journal of stem cells · 2016Review
- Tooth Organ Bioengineering: Cell Sources and Innovative Approaches.Dentistry journal · 2016Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Induced pluripotent stem (iPS) cells are generated from adult cells and are potentially of great value in regenerative medicine. Recently, it was shown that iPS cells can differentiate into ameloblast-like cells in cultures using feeder cells. In the present study, we sought to induce differentiation of ameloblast-like cells from iPS cells under feeder-free conditions using medium conditioned by cultured epithelial cell rests of Malassez (ERM) cells and gelatin-coated dishes. Two culture conditions were compared: co-cultures of iPS cells and ERM cells; and, culture of iPS cells in ERM cell-conditioned medium. Differentiation of ameloblast-like cells in the cultures was assessed using real-time RT-PCR assays of expression of the marker genes keratin 14, amelogenin, and ameloblastin and by immunocytochemical staining for amelogenin. We found greater evidence of ameloblast-like cell differentiation in the cultures using the conditioned medium. In the latter, the level of amelogenin expression increased daily and was significantly higher than controls on the 7th, 10th, and 14th days. Expression of ameloblastin also increased daily and was significantly higher than controls on the 14th day. The present study demonstrates that mouse iPS cells can be induced to differentiate into ameloblast-like cells in feeder-free cell cultures using ERM cell-conditioned medium and gelatin-coated dishes.
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