ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2014
The matrix protein Hikaru genki localizes to cholinergic synaptic clefts and regulates postsynaptic organization in the Drosophila brain.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 14 citations in OpenAlex.
- Motor neurons integrate cholinergic inputs through spatial organization of diverse nicotinic receptors.PNAS nexus · 2026Article
- Quiescent neural stem cells transiently become neuron-like to coordinate long-range reactivation.The EMBO journal · 2026Article
- Muscle cofilin alters neuromuscular junction postsynaptic development to strengthen functional neurotransmission.Development (Cambridge, England) · 2024Article
- Control of Synaptic Levels of Nicotinic Acetylcholine Receptor by the Sequestering Subunit Dα5 and Secreted Scaffold Protein Hig.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Synapse Formation and Function Across Species: Ancient Roles for CCP, CUB, and TSP-1 Structural Domains.Frontiers in neuroscience · 2022Review
- Temporal regulation of nicotinic acetylcholine receptor subunits supports central cholinergic synapse development inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Constructing and Tuning Excitatory Cholinergic Synapses: The Multifaceted Functions of Nicotinic Acetylcholine Receptors inFrontiers in cellular neuroscience · 2021Review
- Serotonergic Modulation of Aggression in Drosophila Involves GABAergic and Cholinergic Opposing Pathways.Current biology : CB · 2019Article
- Cellular diversity in theeLife · 2018Article
- Sociability and synapse subtype-specific defects in mice lacking SRPX2, a language-associated gene.PloS one · 2018Article
- The Matrix Proteins Hasp and Hig Exhibit Segregated Distribution within Synaptic Clefts and Play Distinct Roles in Synaptogenesis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Transmission, Development, and Plasticity of Synapses.Genetics · 2015Review
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3 authors at 1 institution in 1 country.
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Abstract
The synaptic cleft, a crucial space involved in neurotransmission, is filled with extracellular matrix that serves as a scaffold for synaptic differentiation. However, little is known about the proteins present in the matrix and their functions in synaptogenesis, especially in the CNS. Here, we report that Hikaru genki (Hig), a secreted protein with an Ig motif and complement control protein domains, localizes specifically to the synaptic clefts of cholinergic synapses in the Drosophila CNS. The data indicate that this specific localization is achieved by capture of secreted Hig in synaptic clefts, even when it is ectopically expressed in glia. In the absence of Hig, the cytoskeletal scaffold protein DLG accumulated abnormally in cholinergic postsynapses, and the synaptic distribution of acetylcholine receptor (AchR) subunits Dα6 and Dα7 significantly decreased. hig mutant flies consistently exhibited resistance to the AchR agonist spinosad, which causes lethality by specifically activating the Dα6 subunit, suggesting that loss of Hig compromises the cholinergic synaptic activity mediated by Dα6. These results indicate that Hig is a specific component of the synaptic cleft matrix of cholinergic synapses and regulates their postsynaptic organization in the CNS.
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