ArticleMolecular biology of the cell2014
Genetic suppression of a phosphomimic myosin II identifies system-level factors that promote myosin II cleavage furrow accumulation.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 15 citations in OpenAlex.
- A continuum model of mechanosensation based on contractility kit assembly.Biophysical journal · 2025Article
- Nonmuscle myosin IIB is a driver of cellular reprogramming.Molecular biology of the cell · 2023Article
- The lectin Discoidin I acts in the cytoplasm to help assemble the contractile machinery.The Journal of cell biology · 2022Article
- Cancer as a biophysical disease: Targeting the mechanical-adaptability program.Biophysical journal · 2022Review
- The Unusual Suspects in Cytokinesis: Fitting the Pieces Together.Frontiers in cell and developmental biology · 2020Review
- Myosin IIB assembly state determines its mechanosensitive dynamics.The Journal of cell biology · 2019Article
- Contractility kits promote assembly of the mechanoresponsive cytoskeletal network.Journal of cell science · 2019Article
- Parallel Compression Is a Fast Low-Cost Assay for the High-Throughput Screening of Mechanosensory Cytoskeletal Proteins in Cells.ACS applied materials & interfaces · 2017Article
- Mechanochemical Signaling Directs Cell-Shape Change.Biophysical journal · 2017Review
- Cytokinesis: Robust cell shape regulation.Seminars in cell & developmental biology · 2016Review
- Cell shape regulation through mechanosensory feedback control.Journal of the Royal Society, Interface · 2015Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
How myosin II localizes to the cleavage furrow in Dictyostelium and metazoan cells remains largely unknown despite significant advances in understanding its regulation. We designed a genetic selection using cDNA library suppression of 3xAsp myosin II to identify factors involved in myosin cleavage furrow accumulation. The 3xAsp mutant is deficient in bipolar thick filament assembly, fails to accumulate at the cleavage furrow, cannot rescue myoII-null cytokinesis, and has impaired mechanosensitive accumulation. Eleven genes suppressed this dominant cytokinesis deficiency when 3xAsp was expressed in wild-type cells. 3xAsp myosin II's localization to the cleavage furrow was rescued by constructs encoding rcdBB, mmsdh, RMD1, actin, one novel protein, and a 14-3-3 hairpin. Further characterization showed that RMD1 is required for myosin II cleavage furrow accumulation, acting in parallel with mechanical stress. Analysis of several mutant strains revealed that different thresholds of myosin II activity are required for daughter cell symmetry than for furrow ingression dynamics. Finally, an engineered myosin II with a longer lever arm (2xELC), producing a highly mechanosensitive motor, could also partially suppress the intragenic 3xAsp. Overall, myosin II accumulation is the result of multiple parallel and partially redundant pathways that comprise a cellular contractility control system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.