ArticleMolecular microbiology2015
Single-molecule tracking in live Vibrio cholerae reveals that ToxR recruits the membrane-bound virulence regulator TcpP to the toxT promoter.
Article in Molecular microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 54 citations in OpenAlex.
- H-NOX and NosP Regulate Flagellar Protein and Virulence Factor Production in Vibrio Cholerae.Molecular & cellular proteomics : MCP · 2026Article
- Low-affinity DNA-binding promotes cooperative activation of natural transformation inJournal of bacteriology · 2026Article
- Identification and characterization ofFrontiers in microbiology · 2026Article
- Two transmembrane transcriptional regulators coordinate to activate chitin-induced natural transformation in Vibrio cholerae.PLoS genetics · 2025Article
- Achieving Single-Molecule Tracking of Subcellular Regulation in Bacteria during Real-Time Environmental Perturbations.Analytical chemistry · 2023Article
- Advances in cholera research: from molecular biology to public health initiatives.Frontiers in microbiology · 2023Review
- Bile Salts Promote ToxR Regulon Activation during Growth under Virulence-Inducing Conditions.Infection and immunity · 2021Article
- Independent Promoter Recognition by TcpP Precedes Cooperative Promoter Activation by TcpP and ToxR.mBio · 2021Article
- Computerized fluorescence microscopy of microbial cells.World journal of microbiology & biotechnology · 2021Review
- SMAUG: Analyzing single-molecule tracks with nonparametric Bayesian statistics.Methods (San Diego, Calif.) · 2021Article
- Host stimuli and operator binding sites controlling protein interactions between virulence master regulator ToxR and ToxS in Vibrio cholerae.Molecular microbiology · 2020Article
- The 58th Cysteine of TcpP Is Essential forFrontiers in microbiology · 2020Article
- The wing of the ToxR winged helix-turn-helix domain is required for DNA binding and activation of toxT and ompU.PloS one · 2019Article
- DNA-binding directs the localization of a membrane-integrated receptor of the ToxR family.Communications biology · 2019Article
- SMTracker: a tool for quantitative analysis, exploration and visualization of single-molecule tracking data reveals highly dynamic binding of B. subtilis global repressor AbrB throughout the genome.Scientific reports · 2018Article
- Review
- Regulatory Hierarchies Controlling Virulence Gene Expression inFrontiers in microbiology · 2018Review
- Control of virulence gene transcription by indirect readout in Vibrio cholerae and Salmonella enterica serovar Typhimurium.Environmental microbiology · 2017Review
- Robust nonparametric quantification of clustering density of molecules in single-molecule localization microscopy.PloS one · 2017Article
- Calcium Enhances Bile Salt-Dependent Virulence Activation in Vibrio cholerae.Infection and immunity · 2017Article
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4 authors at 2 institutions in 1 country.
Funding
Abstract
Vibrio cholerae causes the human disease cholera by producing a potent toxin. The V. cholerae virulence pathway involves an unusual transcription step: the bitopic inner-membrane proteins TcpP and ToxR activate toxT transcription. As ToxT is the primary direct transcription activator in V. cholerae pathogenicity, its regulation by membrane-localized activators is key in the disease process. However, the molecular mechanisms by which membrane-localized activators engage the transcription process have yet to be uncovered in live cells. Here we report the use of super-resolution microscopy, single-molecule tracking, and gene knockouts to examine the dynamics of individual TcpP proteins in live V. cholerae cells with < 40 nm spatial resolution on a 50 ms timescale. Single-molecule trajectory analysis reveals that TcpP diffusion is heterogeneous and can be described by three populations of TcpP motion: one fast, one slow, and one immobile. By comparing TcpP diffusion in wild-type V. cholerae to that in mutant strains lacking either toxR or the toxT promoter, we determine that TcpP mobility is greater in the presence of its interaction partners than in their absence. Our findings support a mechanism in which ToxR recruits TcpP to the toxT promoter for transcription activation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.