ArticleMemorias do Instituto Oswaldo Cruz2014
Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection.
Article in Memorias do Instituto Oswaldo Cruz, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- The prevalence and role of human respiratory syncytial virus in pediatric respiratory tract infections: a systematic review and meta-analysis of global data.EClinicalMedicine · 2026Article
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- Viral co-detection of influenza virus and other respiratory viruses in hospitalized Brazilian patients during the first three years of the coronavirus disease (COVID)-19 pandemic: an epidemiological profile.Frontiers in microbiology · 2024Article
- Application of MRT-qPCR for pathogen detection of lower respiratory tract infection.American journal of translational research · 2022Article
- Biosensors for the detection of respiratory viruses: A review.Talanta open · 2020Review
- Evaluation of TaqMan Array card (TAC) for the detection of 28 respiratory pathogens.BMC infectious diseases · 2020Article
- Molecular and Immunological Diagnostic Techniques of Medical Viruses.International journal of microbiology · 2020Review
- Molecular Diagnosis of Pneumonia Using Multiplex Real-Time PCR Assay RespiFinder® SMART 22 FAST in a Group of Moroccan Infants.Advances in virology · 2020Article
- Molecular detection of respiratory pathogens among children aged younger than 5 years hospitalized with febrile acute respiratory infections: A prospective hospital-based observational study in Niamey, Niger.Health science reports · 2019Article
- Respiratory Pathogens in Infants Diagnosed with Acute Lower Respiratory Tract Infection in a Tertiary Care Hospital of Western India Using Multiplex Real Time PCR.Indian journal of pediatrics · 2019Article
- Clinical characteristics and viral etiologies of outpatients with acute respiratory infections in Huzhou of China: a retrospective study.BMC infectious diseases · 2019Article
- Bacteriological Study Among Influenza-like Illness Cases in a Community Setting in Pune, India.Cureus · 2018Article
- Bacterial and viral pathogen spectra of acute respiratory infections in under-5 children in hospital settings in Dhaka city.PloS one · 2017Article
- Article
- Utility of multiplex real-time PCR for diagnosing paediatric acute respiratory tract infection in a tertiary care hospital.Medical journal, Armed Forces IndiaArticle
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viruses are the major contributors to the morbidity and mortality of upper and lower acute respiratory infections (ARIs) for all age groups. The aim of this study was to determine the frequencies for a large range of respiratory viruses using a sensitive molecular detection technique in specimens from outpatients of all ages with ARIs. Nasopharyngeal aspirates were obtained from 162 individuals between August 2007-August 2009. Twenty-three pathogenic respiratory agents, 18 respiratory viruses and five bacteria were investigated using multiplex real-time reverse transcriptase polymerase chain reaction (RT-PCR) and indirect immunofluorescence assay (IIF). Through IIF, 33 (20.4%) specimens with respiratory virus were recognised, with influenza virus representing over half of the positive samples. Through a multiplex real-time RT-PCR assay, 88 (54.3%) positive samples were detected; the most prevalent respiratory viral pathogens were influenza, human rhinovirus and respiratory syncytial virus (RSV). Six cases of viral co-detection were observed, mainly involving RSV. The use of multiplex real-time RT-PCR increased the viral detection by 33.9% and revealed a larger number of respiratory viruses implicated in ARI cases, including the most recently described respiratory viruses [human bocavirus, human metapneumovirus, influenza A (H1N1) pdm09 virus, human coronavirus (HCoV) NL63 and HCoV HKU1].
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