ArticleNutrition & metabolism2014
Vitamin D deficiency down-regulates Notch pathway contributing to skeletal muscle atrophy in old wistar rats.
Article in Nutrition & metabolism, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 2 of them syntheses that pooled it.
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Who cites it
29 citing papers in PubMed, 2 syntheses or guidelines pooled it, 66 citations in OpenAlex.
- The Role of Vitamin D in Skeletal Muscle Repair and Regeneration in Animal Models and Humans: A Systematic Review.Nutrients · 2023Pooled it
- Pooled it
- Association of serum 25-hydroxyvitamin D concentrations with premature mortality in adult U.S. patients with sarcopenia.BMC public health · 2026Article
- Cholecalciferol and muscle strength in hemodialysis patients: results from the randomized VITADIAL trial.Clinical kidney journal · 2026Article
- Ubiquitin Ligases in pro-atrophic and antiatrophic signaling cascades in muscles.Molecular biology reports · 2026Review
- Vitamin D Status and Supplementation and the Functional Outcomes of Human Musculoskeletal Tissues: A Stratified Systematic Review.Health science reports · 2026Review
- Malnutrition-Sarcopenia Syndrome in older adults: Causes, consequences, and countermeasures.EXCLI journal · 2026Review
- Regulatory role of vitamin DScientific reports · 2025Article
- Dietary Vitamin D Supplementation Improves Hair Follicle Development and Affects Fatty Acid Metabolism in Rex Rabbits.Food science & nutrition · 2025Article
- Combined exercise-induced modulation of Notch pathway and muscle quality in senescence-accelerated mice.Pflugers Archiv : European journal of physiology · 2025Article
- Notch signaling pathway modulation by food functional ingredients: mechanisms, cancer and immunoregulation.Frontiers in cell and developmental biology · 2025Review
- Nutritional insights into pulmonary fibrosis: a comprehensive review on the impact of vitamins.Frontiers in nutrition · 2025Review
- Characteristics of the gut microbiome and metabolic profile in elderly patients with sarcopenia.Frontiers in pharmacology · 2023Article
- Sirt1 overexpression improves senescence-associated pulmonary fibrosis induced by vitamin D deficiency through downregulating IL-11 transcription.Aging cell · 2022Article
- Review
- Risk Factors Affecting Muscle Mass Decline in Maintenance Hemodialysis Patients.BioMed research international · 2022Article
- Partial-Methylated HeyL Promoter Predicts the Severe Illness in Egyptian COVID-19 Patients.Disease markers · 2022Article
- Deletion of RBP-Jkappa gene in mesenchymal cells causes rickets like symptoms in the mouse.Current medicine (Cham, Switzerland) · 2022Article
- Current Thoughts of Notch's Role in Myoblast Regulation and Muscle-Associated Disease.International journal of environmental research and public health · 2021Review
- Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe diminished ability of aged muscle to self-repair is a factor behind sarcopenia and contributes to muscle atrophy. Muscle repair depends on satellite cells whose pool size is diminished with aging. A reduction in Notch pathway activity may explain the age-related decrease in satellite cell proliferation, as this pathway has been implicated in satellite cell self-renewal. Skeletal muscle is a target of vitamin D which modulates muscle cell proliferation and differentiation in vitro and stimulates muscle regeneration in vivo. Vitamin D status is positively correlated to muscle strength/function, and elderly populations develop a vitamin D deficiency. The aim of this study was to evaluate how vitamin D deficiency induces skeletal muscle atrophy in old rats through a reduction in Notch pathway activity and proliferation potential in muscle.
methods15-month-old male rats were vitamin D-depleted or not (control) for 9 months (n = 10 per group). Rats were 24-month-old at the end of the experiment. Gene and/or protein expression of markers of proliferation, or modulating proliferation, and of Notch signalling pathway were studied in the tibialis anterior muscle by qPCR and western blot. An unpaired student's t-test was performed to test the effect of the experimental conditions.
resultsVitamin D depletion led to a drop in concentrations of plasma 25-hydroxyvitamin D in depleted rats compared to controls (-74%, p < 0.01). Tibialis anterior weight was decreased in D-depleted rats (-25%, p < 0.05). The D-depleted group showed -39%, -31% drops in expression of two markers known to modulate proliferation (Bmp4, Fgf-2 mRNA levels) and -56% drop in one marker of cell proliferation (PCNA protein expression) compared to controls (p < 0.05). Notch pathway activity was blunted in tibialis anterior of D-depleted rats compared to controls, seen as a down-regulation of cleaved Notch (-53%, p < 0.05) and its target Hes1 (-35%, p < 0.05).
conclusionsA 9-month vitamin D depletion induced vitamin D deficiency in old rats. Vitamin D depletion induces skeletal muscle atrophy in old rats through a reduction in Notch pathway activity and proliferation potential. Vitamin D deficiency could aggravate the age-related decrease in muscle regeneration capacity.
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