ArticleApplied physics letters2014
Accurate measurement of force and displacement with optical tweezers using DNA molecules as metrology standards.
Article in Applied physics letters, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Methods for Studying Motor-Driven Viral DNA Packaging in Bacteriophages phi29, Lambda, and T4 via Single DNA Molecule Manipulation and Rapid Solution Exchange.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Role of DNA-DNA sliding friction and nonequilibrium dynamics in viral genome ejection and packaging.Nucleic acids research · 2023Article
- Modeling multiple duplex DNA attachments in a force-extension experiment.Biophysical reports · 2022Article
- Determining Trap Compliances, Microsphere Size Variations, and Response Linearities in Single DNA Molecule Elasticity Measurements with Optical Tweezers.Frontiers in molecular biosciences · 2021Article
- Function of a viral genome packaging motor from bacteriophage T4 is insensitive to DNA sequence.Nucleic acids research · 2020Article
- Functional Dissection of a Viral DNA Packaging Machine's Walker B Motif.Journal of molecular biology · 2019Article
- Nucleotide-dependent DNA gripping and an end-clamp mechanism regulate the bacteriophage T4 viral packaging motor.Nature communications · 2018Article
- Walker-A Motif Acts to Coordinate ATP Hydrolysis with Motor Output in Viral DNA Packaging.Journal of molecular biology · 2016Article
- Continuous allosteric regulation of a viral packaging motor by a sensor that detects the density and conformation of packaged DNA.Biophysical journal · 2015Article
- Repulsive DNA-DNA interactions accelerate viral DNA packaging in phage Phi29.Physical review letters · 2014Article
- Evidence for an electrostatic mechanism of force generation by the bacteriophage T4 DNA packaging motor.Nature communications · 2014Article
- Nonequilibrium dynamics and ultraslow relaxation of confined DNA during viral packaging.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Optical tweezers facilitate measurement of piconewton-level forces and nanometer-level displacements and have broad applications in biophysics and soft matter physics research. We have shown previously that DNA molecules can be used as metrology standards to define such measurements. Force-extension measurements on two DNA molecules of different lengths can be used to determine four necessary measurement parameters. Here, we show that the accuracy of determining these parameters can be improved by more than 7-fold by incorporating measurements of the DNA overstretching transition and using a multi-step data analysis procedure. This method results in very robust and precise fitting of DNA force-extension measurements to the worm-like chain model. We verify the accuracy through independent measurements of DNA stretching, DNA unzipping, and microsphere contact forces.
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Registered trials
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