ReviewDeutsches Arzteblatt international2014
The role of multiplex PCR in respiratory tract infections in children.
Review in Deutsches Arzteblatt international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The trial behind it
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Who cites it
36 citing papers in PubMed, 76 citations in OpenAlex.
- Prevalence and Clinical Impact of Respiratory Viral Infections from the STOP2 Study of Cystic Fibrosis Pulmonary Exacerbations.Annals of the American Thoracic Society · 2024Trial
- Pneumococcal Detection and Bacterial Co-Detection in Children After COVID-19: A Two-Year Multiplex PCR Study.Biomedicines · 2026Article
- Clinical validation of a novel multiplex real-time PCR for diagnosing community-acquired pneumonia in the emergency department: a promising diagnostic tool forFrontiers in microbiology · 2026Article
- Epidemiology and Clinical Features of Respiratory Viruses in Hospitalized Iranian Children During the COVID-19 Pandemic.Immunity, inflammation and disease · 2025Article
- Article
- Digital Melting Curve Analysis for Multiplex Quantification of Nucleic Acids on Droplet Digital PCR.Biosensors · 2025Article
- Temporal dynamics and forecasting of respiratory viral infections during and after the SARS-CoV-2 pandemic (2020-2027): a multiplex PCR and ARIMA-based study.Frontiers in microbiology · 2025Article
- Usefulness of Point-of-Care Testing for Respiratory Viruses in a Pediatric Emergency Department Setting.Journal of clinical medicine · 2024Article
- Chitosan-Based Nanocarriers for Pulmonary and Intranasal Drug Delivery Systems: A Comprehensive Overview of their Applications.Current drug targets · 2024Review
- Viral Etiology and Clinical Characteristics of Acute Respiratory Tract Infections in Hospitalized Children in Southern Germany (2014-2018).Open forum infectious diseases · 2023Article
- Viral infection in chronic otitis media with effusion in children.Frontiers in pediatrics · 2023Article
- Assessment of surveillance predictors for suspected respiratory syncytial virus, influenza andIJID regions · 2022Article
- Usefulness of rapid molecular tests in pediatric respiratory tract infections.Italian journal of pediatrics · 2022Article
- Evaluation of viral respiratory pathogens in children aged under five hospitalized with lower respiratory tract infections.Northern clinics of Istanbul · 2022Article
- Increased Detection of Viruses in Children with Respiratory Tract Infection Using PCR.International journal of environmental research and public health · 2020Article
- The impact analysis of a multiplex PCR respiratory panel for hospitalized pediatric respiratory infections in Japan.Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy · 2020Article
- Association between multiple respiratory viral infections and pediatric intensive care unit admission among infants with bronchiolitis.Archives de pediatrie : organe officiel de la Societe francaise de pediatrie · 2020Article
- Viral respiratory infections diagnosed by multiplex polymerase chain reaction in pediatric patients.Journal of medical virology · 2019Article
- Detection of six common human paramyxoviruses in patients with acute febrile respiratory symptoms using a novel multiplex real-time RT-PCR assay.Journal of medical virology · 2019Article
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInfants, toddlers, and children of primary-school age without any special risk factors generally have three to ten febrile respiratory infections per year. Most such infections are of viral origin and self-limiting, but viral infection is often hard to distinguish from bacterial infection. The use of a multiplex polymerase chain reaction (PCR) to detect viruses in respiratory secretions is potentially beneficial, as it might help physicians avoid giving antibiotics unnecessarily.
methodThis article is based on a selective review of the literature and on the findings of the authors' own investigations.
resultsMultiplex PCR is a highly sensitive, highly specific test for the detection of viral nucleic acids in respiratory secretions. If PCR reveals the presence of RNA derived from respiratory syncytial virus, human metapneumovirus, parainfluenza virus, or influenza virus, then an acute infection caused by the corresponding pathogen is probably present, and further treatment can be given accordingly. On the other hand, the nucleic acids of adeno-, boca-, rhino- or coronaviruses can be found in relatively trivial infections as well as in asymptomatic persons, probably reflecting either a prior infection or a current subclinical one. For children in particular, upper respiratory infections are so common in the winter months that acute and prior infections with these pathogens cannot be distinguished by multiplex PCR. The use of multiplex PCR in children has not been shown to shorten hospital stays or to lessen antibiotic consumption or overall cost.
conclusionThe detectability of viral nucleic acids is an important contribution to the diagnostic assessment of children with severe respiratory infection. For these highly sensitive diagnostic tests to be used optimally, primary viral infections must be distinguished from bacterial superinfections.
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