ArticleEpidemiology and infection2015
The utility and public health implications of PCR and whole genome sequencing for the detection and investigation of an outbreak of Shiga toxin-producing Escherichia coli serogroup O26:H11.
Article in Epidemiology and infection, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Increased detection of Shiga toxin-producingEpidemiology and infection · 2025Article
- A preliminary study of the use of MinION sequencing to specifically detect Shiga toxin-producing Escherichia coli in culture swipes containing multiple serovars of this species.Scientific reports · 2023Article
- Epidemiological investigations identified an outbreak of Shiga toxin-producingEpidemiology and infection · 2021Article
- Investigation of the Causes of ShigatoxigenicMicroorganisms · 2020Article
- Rapid, multiplexed, whole genome and plasmid sequencing of foodborne pathogens using long-read nanopore technology.Scientific reports · 2019Article
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- Attack of the clones: whole genome-based characterization of two closely related enterohemorrhagic Escherichia coli O26 epidemic lineages.BMC genomics · 2018Article
- Setup, Validation, and Quality Control of a Centralized Whole-Genome-Sequencing Laboratory: Lessons Learned.Journal of clinical microbiology · 2018Article
- Critical evaluation of FDA-approved respiratory multiplex assays for public health surveillance.Expert review of molecular diagnostics · 2018Review
- Molecular characterisation of human Shiga toxin-producingEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2017Article
- Real-time genomic investigation underlying the public health response to a Shiga toxin-producing Escherichia coli O26:H11 outbreak in a nursery.Epidemiology and infection · 2017Article
- Whole-Genome Sequencing of Bacterial Pathogens: the Future of Nosocomial Outbreak Analysis.Clinical microbiology reviews · 2017Review
- Virulence Gene Profiles and Clonal Relationships of Escherichia coli O26:H11 Isolates from Feedlot Cattle as Determined by Whole-Genome Sequencing.Applied and environmental microbiology · 2016Article
- Detection, Characterization, and Typing of Shiga Toxin-Producing Escherichia coli.Frontiers in microbiology · 2016Review
- Development of a High Resolution Virulence Allelic Profiling (HReVAP) Approach Based on the Accessory Genome of Escherichia coli to Characterize Shiga-Toxin Producing E. coli (STEC).Frontiers in microbiology · 2016Article
- First step in using molecular data for microbial food safety risk assessment; hazard identification of Escherichia coli O157:H7 by coupling genomic data with in vitro adherence to human epithelial cells.International journal of food microbiology · 2015Article
- Utility of Whole-Genome Sequencing of Escherichia coli O157 for Outbreak Detection and Epidemiological Surveillance.Journal of clinical microbiology · 2015Article
- Whole-Genome Sequencing Data for Serotyping Escherichia coli-It's Time for a Change!Journal of clinical microbiology · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many serogroups of Shiga toxin-producing Escherichia coli (STEC) other than serogroup O157 (non-O157 STEC), for example STEC O26:H11, are highly pathogenic and capable of causing haemolytic uraemic syndrome. A recent increase in non-O157 STEC cases identified in England, resulting from a change in the testing paradigm, prompted a review of the current methods available for detection and typing of non-O157 STEC for surveillance and outbreak investigations. Nineteen STEC O26:H11 strains, including four from a nursery outbreak were selected to assess typing methods. Serotyping and multilocus sequence typing were not able to discriminate between the stx-producing strains in the dataset. However, genome sequencing provided rapid and robust confirmation that isolates of STEC O26:H11 associated with a nursery outbreak were linked at the molecular level, had a common source and were distinct from the other strains analysed. Virulence gene profiling of DNA extracted from a polymerase chain reaction (PCR)-positive/culture-negative faecal specimen from a case that was epidemiologically linked to the STEC O26:H11 nursery outbreak, provided evidence at the molecular level to support that link. During this study, we describe the utility of PCR and the genome sequencing approach in facilitating surveillance and enhancing the response to outbreaks of non-O157 STEC.
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