ArticleJournal of experimental botany2015
Functional diversification of two UGT80 enzymes required for steryl glucoside synthesis in Arabidopsis.
Article in Journal of experimental botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 48 citations in OpenAlex.
- Functional characterization of four glycosyltransferases for biosynthesis of steroidal saponins in medicinal plantSynthetic and systems biotechnology · 2026Article
- Lipidomic signatures of heat tolerance in mungbean [Vigna radiata(L.) Wilczek] reveal reduced fatty acid unsaturation.Scientific reports · 2026Article
- Genome-wide identification of long non-coding RNAs revealing their regulatory roles under seed development in ricebean (Vigna umbellata).BMC plant biology · 2026Article
- Transcriptome and metabolome reveal the accumulation of secondary metabolites in different species of Dioscorea.BMC plant biology · 2025Article
- Sterols in plant biologyCell surface (Amsterdam, Netherlands) · 2025Review
- Potential Utility ofGenes · 2024Article
- The interaction networks of small rubber particle proteins in the latex ofFrontiers in plant science · 2024Article
- A novel sterol glycosyltransferase catalyses steroidal sapogenin 3-O glucosylation from Paris polyphylla var. yunnanensis.Molecular biology reports · 2023Article
- Advances in the Biosynthesis and Molecular Evolution of Steroidal Saponins in Plants.International journal of molecular sciences · 2023Review
- UGT72, a Major Glycosyltransferase Family for Flavonoid and Monolignol Homeostasis in Plants.Biology · 2022Review
- Characterization of UDP-glycosyltransferase family members reveals how major flavonoid glycoside accumulates in the roots of Scutellaria baicalensis.BMC genomics · 2022Article
- Sterol Glucosyltransferases Tailor Polysaccharide Accumulation in Arabidopsis Seed Coat Epidermal Cells.Cells · 2021Article
- Molecular Cloning and Functional Characterization of a Sterol 3-Frontiers in plant science · 2021Article
- Leaf Lipid Alterations in Response to Heat Stress ofPlants (Basel, Switzerland) · 2020Article
- Transcriptome analysis of the endangeredPlant diversity · 2019Article
- Inactivation of UDP-Glucose Sterol Glucosyltransferases EnhancesFrontiers in plant science · 2019Article
- The enzymatic biosynthesis of acylated steroidal glycosides and their cytotoxic activity.Acta pharmaceutica Sinica. B · 2018Article
- Population structure and genetic basis of the agronomic traits of upland cotton in China revealed by a genome-wide association study using high-density SNPs.Plant biotechnology journal · 2017Article
- Positioning of the SCRAMBLED receptor requires UDP-Glc:sterol glucosyltransferase 80B1 in Arabidopsis roots.Scientific reports · 2017Article
- Genome-wide analysis of UDP-glycosyltransferase super family in Brassica rapa and Brassica oleracea reveals its evolutionary history and functional characterization.BMC genomics · 2017Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Steryl glucosides (SG) are abundant steroid conjugates in plant membranes. Beyond structural roles in lipid bilayers, functions in sugar transport, storage, and/or signalling are predicted. UDP-glucose:sterol glucosyltransferase 80A2 (UGT80A2) and UGT80B1, which share similarity to fungal counterparts, are implicated in SG synthesis in Arabidopsis thaliana. A third related enzyme, which seems specific to the plant lineage, is encoded by UGT713B1/At5g24750. Genetic and biochemical approaches were employed to determine the role of each UGT gene in the production of specific SGs and acyl SGs (ASGs). Using direct infusion electrospray ionization tandem mass spectrometry (ESI-MS/MS), SG and acyl SG (ASG) contents of ugt80 and ugt713 mutants, and triple and double mutants were profiled in seeds. In vitro enzyme assays were performed to assay substrate preferences. Both UGT80A2 and UGT80B1, but not UGT713B1 were shown to be coordinately down-regulated during seed imbibition when SG levels decline, consistent with similar functions as UGT80 enzymes. UGT80A2 was found to be required for normal levels of major SGs in seeds, whereas UGT80B1 is involved in accumulation of minor SG and ASG compounds. Although the results demonstrate specific activities for UGT80A2 and UGT80B1, a role for UGT713B1 in SG synthesis was not supported. The data show that UGT80A2, the more highly conserved enzyme, is responsible for the bulk production of SGs in seeds, whereas UGT80B1 plays a critical accessory role. This study extends our knowledge of UGT80 enzymes and provides evidence for specialized functions for distinct classes of SG and ASG molecules in plants.
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