ArticleScientific reports2014
Introduction of impermeable actin-staining molecules to mammalian cells by optoporation.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Polyoxometalates enable cellular uptake of the actin-binding toxin phalloidin.Journal of materials chemistry. B · 2026Article
- Silactins and Structural Diversity of Biosilica in Sponges.Biomimetics (Basel, Switzerland) · 2024Article
- Comprehensive in vitro and in ovo assessment of cytotoxicity: Unraveling the impact of sodium fluoride, xylitol, and their synergistic associations in dental products.Biomolecules & biomedicine · 2024Article
- Boron clusters as broadband membrane carriers.Nature · 2022Article
- Article
- Laser-assisted optoporation of cells and tissues - a mini-review.Biomedical optics express · 2019Article
- Nano-enhanced Optical Gene Delivery to Retinal Degenerated Mice.Current gene therapy · 2019Review
- Advanced physical techniques for gene delivery based on membrane perforation.Drug delivery · 2018Review
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- Ultrafast laser-assisted spatially targeted optoporation into cortical axons and retinal cells in the eye.Journal of biomedical optics · 2017Article
- Integrated Electrowetting Nanoinjector for Single Cell Transfection.Scientific reports · 2016Article
- Software-aided automatic laser optoporation and transfection of cells.Scientific reports · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
The selective insertion of foreign materials, such as fluorescent markers or plasmids, into living cells has been a challenging problem in cell biology due to the cell membrane's selective permeability. However, it is often necessary that researchers insert such materials into cells for various dynamical and/or drug delivery studies. This problem becomes even more challenging if the study is to be limited to specific cells within a larger population, since other transfection methods, such as viral transfection and lipofection, are not realizable with a high degree of spatial selectivity. Here, we have used a focused femtosecond laser beam to create a small transient hole in the cellular membrane (optoporation) in order to inject nanomolar concentrations of rhodamine phalloidin (an impermeable dye molecule for staining filamentous actin) into targeted living mammalian cells (both HEK and primary cortical neurons). Following optoporation, the dye bound to the intracellular actin network and rise in fluorescence intensity was observed. Theoretical dynamics of the dye's diffusion is discussed, and numerical simulations of diffusion time constants are found to match well with experimental values.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.