Evidence map›Paper›PMID 25315601›Full record

ArticlePlant physiology2014

Mutation of the inducible ARABIDOPSIS THALIANA CYTOCHROME P450 REDUCTASE2 alters lignin composition and improves saccharification.

Lisa Sundin, Ruben Vanholme, Jan Geerinck, Geert Goeminne, René Höfer, Hoon Kim, John Ralph, Wout Boerjan

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.

0numbers the graph read from it
0cells of the map it votes in
41citing papers in PubMed
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

41 citing papers in PubMed, 68 citations in OpenAlex.

  1. Article
  2. From Defense Executor to Engineering Target: Harnessing Lignin for Crop Resistance.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Review
  3. Article
  4. Article
  5. GM crops & food · 2024
    Article
  6. Article
  7. Review
  8. Article
  9. Article
  10. Metabolite Profiling of Paraquat TolerantAntioxidants (Basel, Switzerland) · 2022
    Article
  11. Article
  12. Article
  13. CytochromeFrontiers in plant science · 2022
    Review
  14. Article
  15. Article
  16. Review
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Lisa SundinDepartment of Plant Systems Biology, VIB, 9052 Ghent, Belgium (L.S., R.V., J.G., G.G., R.H., W.B.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (L.S., R.V., J.G., G.G., R.H., W.B.); andDepartments of Biochemistry and Biological Systems Engineering and Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin 53726 (H.K., J.R.).
Ruben VanholmeDepartment of Plant Systems Biology, VIB, 9052 Ghent, Belgium (L.S., R.V., J.G., G.G., R.H., W.B.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (L.S., R.V., J.G., G.G., R.H., W.B.); andDepartments of Biochemistry and Biological Systems Engineering and Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin 53726 (H.K., J.R.).
Jan GeerinckDepartment of Plant Systems Biology, VIB, 9052 Ghent, Belgium (L.S., R.V., J.G., G.G., R.H., W.B.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (L.S., R.V., J.G., G.G., R.H., W.B.); andDepartments of Biochemistry and Biological Systems Engineering and Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin 53726 (H.K., J.R.).
Geert GoeminneDepartment of Plant Systems Biology, VIB, 9052 Ghent, Belgium (L.S., R.V., J.G., G.G., R.H., W.B.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (L.S., R.V., J.G., G.G., R.H., W.B.); andDepartments of Biochemistry and Biological Systems Engineering and Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin 53726 (H.K., J.R.).
René HöferDepartment of Plant Systems Biology, VIB, 9052 Ghent, Belgium (L.S., R.V., J.G., G.G., R.H., W.B.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (L.S., R.V., J.G., G.G., R.H., W.B.); andDepartments of Biochemistry and Biological Systems Engineering and Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin 53726 (H.K., J.R.).
Hoon KimDepartment of Plant Systems Biology, VIB, 9052 Ghent, Belgium (L.S., R.V., J.G., G.G., R.H., W.B.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (L.S., R.V., J.G., G.G., R.H., W.B.); andDepartments of Biochemistry and Biological Systems Engineering and Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin 53726 (H.K., J.R.).
John RalphDepartment of Plant Systems Biology, VIB, 9052 Ghent, Belgium (L.S., R.V., J.G., G.G., R.H., W.B.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (L.S., R.V., J.G., G.G., R.H., W.B.); andDepartments of Biochemistry and Biological Systems Engineering and Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin 53726 (H.K., J.R.).
Wout BoerjanDepartment of Plant Systems Biology, VIB, 9052 Ghent, Belgium (L.S., R.V., J.G., G.G., R.H., W.B.);Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (L.S., R.V., J.G., G.G., R.H., W.B.); andDepartments of Biochemistry and Biological Systems Engineering and Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin 53726 (H.K., J.R.) woboe@psb.vib-ugent.be.
Ghent University · BEVIB-UGent Center for Plant Systems Biology · BEUniversity of Wisconsin–Madison · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

ARABIDOPSIS THALIANA CYTOCHROME P450 REDUCTASE1 (ATR1) and ATR2 provide electrons from NADPH to a large number of CYTOCHROME P450 (CYP450) enzymes in Arabidopsis (Arabidopsis thaliana). Whereas ATR1 is constitutively expressed, the expression of ATR2 appears to be induced during lignin biosynthesis and upon stresses. Therefore, ATR2 was hypothesized to be preferentially involved in providing electrons to the three CYP450s involved in lignin biosynthesis: CINNAMATE 4-HYDROXYLASE (C4H), p-COUMARATE 3-HYDROXYLASE1 (C3H1), and FERULATE 5-HYDROXYLASE1 (F5H1). Here, we show that the atr2 mutation resulted in a 6% reduction in total lignin amount in the main inflorescence stem and a compositional shift of the remaining lignin to a 10-fold higher fraction of p-hydroxyphenyl units at the expense of syringyl units. Phenolic profiling revealed shifts in lignin-related phenolic metabolites, in particular with the substrates of C4H, C3H1 and F5H1 accumulating in atr2 mutants. Glucosinolate and flavonol glycoside biosynthesis, both of which also rely on CYP450 activities, appeared less affected. The cellulose in the atr2 inflorescence stems was more susceptible to enzymatic hydrolysis after alkaline pretreatment, making ATR2 a potential target for engineering plant cell walls for biofuel production.

Indexed as

ArabidopsisArabidopsis ProteinsBasic Helix-Loop-Helix ProteinsCelluloseElectronsFlavonolsGlucosinolatesHydrolysisInflorescenceLigninMutationNADPOxidation-ReductionPhenolsPlant StemsProto-Oncogene Proteins c-mybArabidopsis ProteinsATR1 protein, ArabidopsisATR2 protein, ArabidopsisBasic Helix-Loop-Helix ProteinsCelluloseFlavonolsGlucosinolatesLigninNADPPhenolsProto-Oncogene Proteins c-myb

Identifiers

PMID25315601
PMCPMC4256863
OpenAlexW2043118095

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.