ArticleBMC genomics2014
A comprehensive proteomic approach to identifying capacitation related proteins in boar spermatozoa.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 133 citations in OpenAlex.
- Phenotyping of post-fertilization sperm mitophagy determinants discovered in a mammalian gamete-based cell-free system.Biological research · 2026Article
- Garin3 regulates sperm-oocyte fusion and male fertility.Cellular and molecular life sciences : CMLS · 2026Article
- Proteomic signatures of sperm and extracellular vesicles associated with sperm freezability in Holstein bulls.Scientific reports · 2026Article
- Spatiotemporal translation of sperm acrosome associated proteins during early capacitation modulates sperm fertilizing ability.Journal of advanced research · 2026Article
- Progress of sex control techniques in mammals†.Biology of reproduction · 2025Review
- Effect of Capacitation on Proteomic Profile and Mitochondrial Parameters of Spermatozoa in Bulls.Journal of proteome research · 2025Article
- Proteomic analysis reveals the difference between the spermatozoa of young and old Sus scrofa.Scientific reports · 2025Article
- Proteomic and functional analysis of alpaca (Frontiers in veterinary science · 2025Article
- Functional characterization and phenotyping of RAB2A and Lactadherin/MFGE8 as boar sperm zona pellucida binding proteins.Frontiers in cell and developmental biology · 2025Article
- RNA profiles differ between small and large extracellular vesicle subsets isolated from porcine seminal plasma.BMC genomics · 2024Article
- A Comparison of White and Yellow Seminal Plasma Phosphoproteomes Obtained from Turkey (International journal of molecular sciences · 2024Article
- Bottom-up approach to deciphering the targets of the ubiquitin-proteasome system in porcine sperm capacitation.Scientific reports · 2024Article
- Comparative Proteomic Identification of Ram Sperm before and after In Vitro Capacitation.Animals : an open access journal from MDPI · 2024Article
- Proteomic Insights into Seminal Plasma and Spermatozoa Proteins of Small-Spotted Catsharks,Animals : an open access journal from MDPI · 2024Article
- Article
- Prediction of male fertility using Ras-related proteins.Journal of animal science and technology · 2022Article
- Unbalanced Expression of Glutathione Peroxidase 4 and Arachidonate 15-Lipoxygenase Affects Acrosome Reaction and In Vitro Fertilization.International journal of molecular sciences · 2022Article
- Establishment of a male fertility prediction model with sperm RNA markers in pigs as a translational animal model.Journal of animal science and biotechnology · 2022Article
- The ribosome inhibitor chloramphenicol induces motility deficits in human spermatozoa: A proteomic approach identifies potentially involved proteins.Frontiers in cell and developmental biology · 2022Article
- Novaluron Has Detrimental Effects on Sperm Functions.International journal of environmental research and public health · 2021Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMammalian spermatozoa must undergo capacitation, before becoming competent for fertilization. Despite its importance, the fundamental molecular mechanisms of capacitation are poorly understood. Therefore, in this study, we applied a proteomic approach for identifying capacitation-related proteins in boar spermatozoa in order to elucidate the events more precisely. 2-DE gels were generated from spermatozoa samples in before- and after-capacitation. To validate the 2-DE results, Western blotting and immunocytochemistry were performed with 2 commercially available antibodies. Additionally, the protein-related signaling pathways among identified proteins were detected using Pathway Studio 9.0.
resultWe identified Ras-related protein Rab-2, Phospholipid hydroperoxide glutathione peroxidase (PHGPx) and Mitochondrial pyruvate dehydrogenase E1 component subunit beta (PDHB) that were enriched before-capacitation, and NADH dehydrogenase 1 beta subcomplex 6, Mitochondrial peroxiredoxin-5, (PRDX5), Apolipoprotein A-I (APOA1), Mitochondrial Succinyl-CoA ligase [ADP-forming] subunit beta (SUCLA2), Acrosin-binding protein, Ropporin-1A, and Spermadhesin AWN that were enriched after-capacitation (>3-fold) by 2-DE and ESI-MS/MS. SUCLA2 and PDHB are involved in the tricarboxylic acid cycle, whereas PHGPx and PRDX5 are involved in glutathione metabolism. SUCLA2, APOA1 and PDHB mediate adipocytokine signaling and insulin action. The differentially expressed proteins following capacitation are putatively related to sperm functions, such as ROS and energy metabolism, motility, hyperactivation, the acrosome reaction, and sperm-egg interaction.
conclusionThe results from this study elucidate the proteins involved in capacitation, which may aid in the design of biomarkers that can be used to predict boar sperm quality.
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