ArticlePhysiological genomics2014
TNF-α alters the release and transfer of microparticle-encapsulated miRNAs from endothelial cells.
Article in Physiological genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 64 citations in OpenAlex.
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- A change of heart: understanding the mechanisms regulating cardiac proliferation and metabolism before and after birth.The Journal of physiology · 2023Review
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- Extracellular vesicles in β cell biology: Role of lipids in vesicle biogenesis, cargo, and intercellular signaling.Molecular metabolism · 2022Review
- Microparticles: biogenesis, characteristics and intervention therapy for cancers in preclinical and clinical research.Journal of nanobiotechnology · 2022Review
- miRetrieve-an R package and web application for miRNA text mining.NAR genomics and bioinformatics · 2021Article
- Diversity of Lipid Function in Atherogenesis: A Focus on Endothelial Mechanobiology.International journal of molecular sciences · 2021Review
- MicroRNA regulation of cholesterol metabolism.Annals of the New York Academy of Sciences · 2021Review
- The Impact of microRNAs in Renin-Angiotensin-System-Induced Cardiac Remodelling.International journal of molecular sciences · 2021Review
- Circulating argonaute-bound microRNA-126 reports vascular dysfunction and treatment response in acute and chronic kidney disease.iScience · 2021Article
- Extracellular Vesicles Derived from a Human Brain Endothelial Cell Line Increase Cellular ATP Levels.AAPS PharmSciTech · 2021Article
- MiR-502 Suppresses TNF-BioMed research international · 2021Article
- Changes in circulating microRNA and arterial stiffness following high-intensity interval and moderate intensity continuous exercise.Physiological reports · 2020Article
- Extracellular Vesicles as Mediators of Cellular Crosstalk Between Immune System and Kidney Graft.Frontiers in immunology · 2020Review
- The Cell Type-Specific Functions of miR-21 in Cardiovascular Diseases.Frontiers in genetics · 2020Review
- Resistin and Cardiac Arrest-A Prospective Study.Journal of clinical medicine · 2019Article
- The role of microvesicles containing microRNAs in vascular endothelial dysfunction.Journal of cellular and molecular medicine · 2019Review
- The effects of moderate and high-intensity exercise on circulating markers of endothelial integrity and activation in young, healthy men.Journal of applied physiology (Bethesda, Md. : 1985) · 2019Article
- MicroRNAs as a Potential Quality Measurement Tool of Platelet Concentrate Stored in Blood Banks-A Review.Cells · 2019Review
- Progressing from Recurring Tissue Injury to Genomic Instability: A New Mechanism of Neutrophil Pathogenesis.DNA and cell biology · 2019Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
MicroRNAs (miRNAs) encapsulated within microparticles (MPs) are likely to have a role in cell-to-cell signaling in a variety of diseases, including atherosclerosis. However, little is known about the mechanisms by which different cell types release and transfer miRNAs. Here, we examined TNF-α-induced release of MP-encapsulated miR-126, miR-21, and miR-155 from human aortic endothelial cells (ECs) and their transfer to recipient cells. ECs were treated with TNF-α (100 ng/ml) in the presence or absence of inhibitors that target different MP production pathways. MPs released in response to TNF-α were characterized by: 1) 70-80% decrease in miRNA/MP levels for miR-126 and -21 but a significant increase in pre-miR-155 and miR-155 (P < 0.05), 2) 50% reduction in uptake by recipient cells (P < 0.05), and 3) diminished ability to transfer miRNA to recipient cells. Cotreatment of donor ECs with TNF-α and caspase inhibitor (Q-VD-OPH, 10 μM) produced MPs that had: 1) 1.5- to 2-fold increase in miRNA/MP loading, 2) enhanced uptake by recipient cells (2-fold), and 3) increased ability to transfer miR-155. Cotreatment of ECs with TNF-α and Rho-associated kinase (ROCK) inhibitor (10 μM) produced MPs with features similar to those produced by TNF-α treatment alone. Our data indicate that TNF-α induced the production of distinct MP populations: ROCK-dependent, miRNA-rich MPs that effectively transferred their cargo and were antiapoptotic, and caspase-dependent, miRNA-poor MPs that were proapoptotic. These data provide insight into the relationship between MP production and extracellular release of miRNA, as well as the potential of encapsulated miRNA for cell-to-cell communication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.