ArticleAngewandte Chemie (International ed. in English)2014
Photocaged DNAzymes as a general method for sensing metal ions in living cells.
Article in Angewandte Chemie (International ed. in English), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 206 citations in OpenAlex.
- Rational Design Strategies for Stimuli-Responsive DNAzymes Using Modified and Artificial Nucleotides.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Review
- Signal Transduction Strategies for DNAzyme-Based Sensing and Imaging of Metal Ions in Cells andChemical & biomedical imaging · 2025Review
- The Programmable Catalytic Core of 8-17 DNAzymes.Molecules (Basel, Switzerland) · 2024Article
- Rectifying artificial nanochannels with multiple interconvertible permeability states.Nature communications · 2024Article
- Mechanochemical Activation of DNAzyme by Ultrasound.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- A Semisynthetic Bioluminescence Sensor for Ratiometric Imaging of Metal Ions In Vivo Using DNAzymes Conjugated to An Engineered Nano-Luciferase.Angewandte Chemie (International ed. in English) · 2023Article
- Ratiometric Detection of ZnChemistry (Basel, Switzerland) · 2023Article
- Functional Nucleic Acid Probes Based on Two-Photon for Biosensing.Biosensors · 2023Review
- A Highly Selective Mn(II)-Specific DNAzyme and Its Application in Intracellular Sensing.JACS Au · 2023Article
- Single-Molecule Study of DNAzyme Reveals Its Intrinsic Conformational Dynamics.International journal of molecular sciences · 2023Article
- Overcoming Major Barriers to Developing Successful Sensors for Practical Applications Using Functional Nucleic Acids.Annual review of analytical chemistry (Palo Alto, Calif.) · 2022Review
- Noninvasive and Spatiotemporal Control of DNAzyme-Based Imaging of Metal IonsJournal of the American Chemical Society · 2022Article
- Photochemical modifications for DNA/RNA oligonucleotides.RSC advances · 2022Review
- Intelligent demethylase-driven DNAzyme sensor for highly reliable metal-ion imaging in living cells.Chemical science · 2021Article
- DNAzyme-Based Lithium-Selective Imaging Reveals Higher Lithium Accumulation in Bipolar Disorder Patient-Derived Neurons.ACS central science · 2021Article
- Biosensing with DNAzymes.Chemical Society reviews · 2021Review
- DNA Assembly-Based Stimuli-Responsive Systems.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Wavelength-Selective Activation of Photocaged DNAzymes for Metal Ion Sensing in Live Cells.ACS omega · 2021Article
- Cell Surface Engineering Using DNAzymes: Metal Ion Mediated Control of Cell-Cell Interactions.Journal of the American Chemical Society · 2021Article
- Insight into an Oxidative DNA-Cleaving DNAzyme: Multiple Cofactors, the Catalytic Core Map and a Highly Efficient Variant.iScience · 2020Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
DNAzymes, which are sequences of DNA with catalytic activity, have been demonstrated as a potential platform for sensing a wide range of metal ions. Despite their significant promise, cellular sensing using DNAzymes has however been difficult, mainly because of the "always-on" mode of first-generation DNAzyme sensors. To overcome this limitation, a photoactivatable (or photocaged) DNAzyme was designed and synthesized, and its application in sensing Zn(II) in living cells was demonstrated. In this design, the adenosine ribonucleotide at the scissile position of the 8-17 DNAzyme was replaced by 2'-O-nitrobenzyl adenosine, rendering the DNAzyme inactive and thus allowing its delivery into cells intact, protected from nonspecific degradation within cells. Irradiation at 365 nm restored DNAzyme activity, thus allowing the temporal control over the sensing activity of the DNAzyme for metal ions. The same strategy was also applied to the GR-5 DNAzyme for the detection of Pb(II), thus demonstrating the possible scope of the method.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.