ArticlePloS one2014
Differentiation of Bacillus pumilus and Bacillus safensis using MALDI-TOF-MS.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Plant Growth-Promoting Bacteria from Tropical Soils: In Vitro Assessment of Functional Traits.Microorganisms · 2025Article
- Enhanced vitamin BBMC biotechnology · 2024Article
- Accurate noise-robust classification of Bacillus species from MALDI-TOF MS spectra using a denoising autoencoder.Journal of integrative bioinformatics · 2023Article
- Use of bacterial binder in repair mortar for micro-crack remediation.Applied microbiology and biotechnology · 2023Article
- Biotic countermeasures that rescueFrontiers in microbiology · 2023Article
- Differentiating betweenFoods (Basel, Switzerland) · 2022Article
- Malignant T Cell Activation by aJID innovations : skin science from molecules to population health · 2022Article
- Changes in Metabolic Regulation and the Microbiota Composition after Supplementation with Different Fatty Acids in db/db Mice.International journal of food science · 2022Article
- Current Classification of the Bacillus pumilus Group Species, the Rubber-Pathogenic Bacteria Causing Trunk Bulges Disease in Malaysia as Assessed by MLSA and Multi rep-PCR Approaches.The plant pathology journal · 2021Article
- Proteomic Characterization of Bacteriophage Peptides from the Mastitis ProducerFoods (Basel, Switzerland) · 2021Article
- Redox-Oligomeric State of Peroxiredoxin-2 and Glyceraldehyde-3-Phosphate Dehydrogenase in Obstructive Sleep Apnea Red Blood Cells under Positive Airway Pressure Therapy.Antioxidants (Basel, Switzerland) · 2020Article
- Whole-Genome Sequences of an Abortive Bacillus safensis Strain Isolated from a Mare's Uterus.Microbiology resource announcements · 2020Article
- Markers to Rapidly DistinguishFrontiers in microbiology · 2020Article
- Characterization of Bacteriophage Peptides of PathogenicFrontiers in microbiology · 2020Article
- Article
- Identification and Classification for theFrontiers in microbiology · 2018Article
- Microbial biodiversity assessment of the European Space Agency's ExoMars 2016 mission.Microbiome · 2017Article
- Genomic and functional features of the biosurfactant producing Bacillus sp. AM13.Functional & integrative genomics · 2016Article
- Bacillus pumilus Septic Arthritis in a Healthy Child.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2016Article
- Identification of oxidoreductases from the petroleumBiotechnology reports (Amsterdam, Netherlands) · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) despite being increasingly used as a method for microbial identification, still present limitations in which concerns the differentiation of closely related species. Bacillus pumillus and Bacillus safensis, are species of biotechnological and pharmaceutical significance, difficult to differentiate by conventional methodologies. In this study, using a well-characterized collection of B. pumillus and B. safensis isolates, we demonstrated the suitability of MALDI-TOF-MS combined with chemometrics to accurately and rapidly identify them. Moreover, characteristic species-specific ion masses were tentatively assigned, using UniProtKB/Swiss-Prot and UniProtKB/TrEMBL databases and primary literature. Delineation of B. pumilus (ions at m/z 5271 and 6122) and B. safensis (ions at m/z 5288, 5568 and 6413) species were supported by a congruent characteristic protein pattern. Moreover, using a chemometric approach, the score plot created by partial least square discriminant analysis (PLSDA) of mass spectra demonstrated the presence of two individualized clusters, each one enclosing isolates belonging to a species-specific spectral group. The generated pool of species-specific proteins comprised mostly ribosomal and SASPs proteins. Therefore, in B. pumilus the specific ion at m/z 5271 was associated with a small acid-soluble spore protein (SASP O) or with 50S protein L35, whereas in B. safensis specific ions at m/z 5288 and 5568 were associated with SASP J and P, respectively, and an ion at m/z 6413 with 50S protein L32. Thus, the resulting unique protein profile combined with chemometric analysis, proved to be valuable tools for B. pumilus and B. safensis discrimination, allowing their reliable, reproducible and rapid identification.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.