ArticleAngewandte Chemie (International ed. in English)2014
Direct evidence for a peroxide intermediate and a reactive enzyme-substrate-dioxygen configuration in a cofactor-free oxidase.
Article in Angewandte Chemie (International ed. in English), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 48 citations in OpenAlex.
- Radiation-induced peroxide rupture and its temperature-dependent repair probed by homogeneous X-ray irradiation.Acta crystallographica. Section D, Structural biology · 2026Article
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- Cofactor-independent C-C bond cleavage reactions catalyzed by the AlpJ family of oxygenases in atypical angucycline biosynthesis.Beilstein journal of organic chemistry · 2024Article
- Neutron crystallographic refinement with REFMAC5 from the CCP4 suite.Acta crystallographica. Section D, Structural biology · 2023Article
- Evolutionary adaptation from hydrolytic to oxygenolytic catalysis at the α/β-hydrolase fold.Chemical science · 2023Article
- Cysteine Enrichment Mediates Co-Option of Uricase in Reptilian Skin and Transition to Uricotelism.Molecular biology and evolution · 2023Article
- Introduction to the virtual thematic issue on room-temperature biological crystallography.IUCrJ · 2023Article
- Spin-Forbidden Addition of Molecular Oxygen to Stable Enol Intermediates-Decarboxylation of 2-Methyl-1-tetralone-2-carboxylic Acid.International journal of molecular sciences · 2023Article
- Introduction to the virtual thematic issue on room-temperature biological crystallography.Acta crystallographica. Section F, Structural biology communications · 2023Article
- Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive.IUCrJ · 2022Article
- Quantifying and comparing radiation damage in the Protein Data Bank.Nature communications · 2022Article
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- Computational Approach for Rational Design of Fusion Uricase with PAS Sequences.International journal of molecular and cellular medicine · 2020Article
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- A role for Vibrio vulnificus PecS during hypoxia.Scientific reports · 2019Article
- Estimate your dose: RADDOSE-3D.Protein science : a publication of the Protein Society · 2018Article
- Catalytic Mechanisms for Cofactor-Free Oxidase-Catalyzed Reactions: Reaction Pathways of Uricase-Catalyzed Oxidation and Hydration of Uric Acid.ACS catalysis · 2017Article
- Choosing the optimal spectroscopic toolkit to understand protein function.Bioscience reports · 2017Article
- Structural analysis of the bright monomeric yellow-green fluorescent protein mNeonGreen obtained by directed evolution.Acta crystallographica. Section D, Structural biology · 2016Article
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9 authors at 4 institutions in 2 countries.
Funding
Abstract
Cofactor-free oxidases and oxygenases promote and control the reactivity of O2 with limited chemical tools at their disposal. Their mechanism of action is not completely understood and structural information is not available for any of the reaction intermediates. Near-atomic resolution crystallography supported by in crystallo Raman spectroscopy and QM/MM calculations showed unambiguously that the archetypical cofactor-free uricase catalyzes uric acid degradation via a C5(S)-(hydro)peroxide intermediate. Low X-ray doses break specifically the intermediate C5-OO(H) bond at 100 K, thus releasing O2 in situ, which is trapped above the substrate radical. The dose-dependent rate of bond rupture followed by combined crystallographic and Raman analysis indicates that ionizing radiation kick-starts both peroxide decomposition and its regeneration. Peroxidation can be explained by a mechanism in which the substrate radical recombines with superoxide transiently produced in the active site.
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