ArticleDevelopmental cell2014
Minus-end-directed Kinesin-14 motors align antiparallel microtubules to control metaphase spindle length.
Article in Developmental cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
- In vitro reconstitution defines the mechanistic basis of HSET motor activity regulation by IntraFlagellar Transport proteins.Communications biology · 2026Article
- Mechanism of spindle stability and poleward flux regulating spindle length during the metaphase.iScience · 2025Article
- The Kinesin-14 tail: Dual microtubule binding domains drive spindle morphogenesis through tight microtubule cross-linking and robust sliding.Molecular biology of the cell · 2025Article
- Force-transducing molecular ensembles at growing microtubule tips control mitotic spindle size.Nature communications · 2024Article
- Kinesin-14 HSET and KlpA are non-processive microtubule motors with load-dependent power strokes.Nature communications · 2024Article
- Positioning centrioles and centrosomes.The Journal of cell biology · 2024Review
- Rapid binding to protofilament edge sites facilitates tip tracking of EB1 at growing microtubule plus-ends.eLife · 2024Article
- Evidence of kinesin motors involved in stable kinetochore assembly during early meiosis.Molecular biology of the cell · 2023Article
- A fine balance among key biophysical factors is required for recovery of bipolar mitotic spindle from monopolar and multipolar abnormalities.Molecular biology of the cell · 2023Article
- Cik1 and Vik1 accessory proteins confer distinct functions to the kinesin-14 Kar3.Journal of cell science · 2023Article
- Systematic analysis of microtubule plus-end networks defines EB-cargo complexes critical for mitosis in budding yeast.Molecular biology of the cell · 2023Article
- Article
- Kinesin-14 motors participate in a force balance at microtubule plus-ends to regulate dynamic instability.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Slk19 enhances cross-linking of microtubules by Ase1 and Stu1.Molecular biology of the cell · 2021Article
- TheInternational journal of molecular sciences · 2021Article
- Microtubule pivoting enables mitotic spindle assembly in S. cerevisiae.The Journal of cell biology · 2021Article
- Distinct retrograde microtubule motor sets drive early and late endosome transport.The EMBO journal · 2020Article
- The EB1-Kinesin-14 complex is required for efficient metaphase spindle assembly and kinetochore bi-orientation.The Journal of cell biology · 2020Article
- Mitotic Poisons in Research and Medicine.Molecules (Basel, Switzerland) · 2020Review
- The Tail of Kinesin-14a in Giardia Is a Dual Regulator of Motility.Current biology : CB · 2020Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
During cell division, a microtubule-based mitotic spindle mediates the faithful segregation of duplicated chromosomes into daughter cells. Proper length control of the metaphase mitotic spindle is critical to this process and is thought to be achieved through a mechanism in which spindle pole separation forces from plus-end-directed motors are balanced by forces from minus-end-directed motors that pull spindle poles together. However, in contrast to this model, metaphase mitotic spindles with inactive kinesin-14 minus-end-directed motors often have shorter spindle lengths, along with poorly aligned spindle microtubules. A mechanistic explanation for this paradox is unknown. Using computational modeling, in vitro reconstitution, live-cell fluorescence microscopy, and electron microscopy, we now find that the budding yeast kinesin-14 molecular motor Kar3-Cik1 can efficiently align spindle microtubules along the spindle axis. This then allows plus-end-directed kinesin-5 motors to efficiently exert the outward microtubule sliding forces needed for proper spindle bipolarity.
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