ArticlePloS one2014
Complementary effects of two growth factors in multifunctionalized silk nanofibers for nerve reconstruction.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 63 citations in OpenAlex.
- Dehydrated silk fibroin matrices as versatile delivery systems for extracellular vesicles.Frontiers in bioengineering and biotechnology · 2026Article
- Recent Advances in Polymeric Drug Delivery Systems for Peripheral Nerve Regeneration.Pharmaceutics · 2023Review
- Evaluation and Comparison of the Effects of Mature Silkworm (Iranian journal of pharmaceutical research : IJPR · 2022Article
- Functionalized 3D-printed silk-hydroxyapatite scaffolds for enhanced bone regeneration with innervation and vascularization.Biomaterials · 2021Article
- Protein and Polysaccharide-Based Electroactive and Conductive Materials for Biomedical Applications.Molecules (Basel, Switzerland) · 2021Review
- Silk Fibroin: An Ancient Material for Repairing the Injured Nervous System.Pharmaceutics · 2021Review
- Defining the regenerative effects of native spider silk fibers on primary Schwann cells, sensory neurons, and nerve-associated fibroblasts.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Advances and clinical challenges for translating nerve conduit technology from bench to bed side for peripheral nerve repair.Cell and tissue research · 2021Review
- The Materiobiology of Silk: Exploring the Biophysical Influence of Silk Biomaterials on Directing Cellular Behaviors.Frontiers in bioengineering and biotechnology · 2021Review
- Growth factors-based therapeutic strategies and their underlying signaling mechanisms for peripheral nerve regeneration.Acta pharmacologica Sinica · 2020Review
- Protein and Polysaccharide-Based Fiber Materials Generated from Ionic Liquids: A Review.Molecules (Basel, Switzerland) · 2020Review
- Novel electrospun poly(ε-caprolactone)/type I collagen nanofiber conduits for repair of peripheral nerve injury.Neural regeneration research · 2019Article
- Development of Oxidized Polyvinyl Alcohol-Based Nerve Conduits Coupled with the Ciliary Neurotrophic Factor.Materials (Basel, Switzerland) · 2019Article
- A multi-layered nerve guidance conduit design adapted to facilitate surgical implantation.Health science reports · 2018Article
- Fabrication of Hemin-Doped Serum Albumin-Based Fibrous Scaffolds for Neural Tissue Engineering Applications.ACS applied materials & interfaces · 2018Article
- Honeysuckle flowers extract loaded Bombyx mori silk fibroin films for inducing apoptosis of HeLa cells.Microscopy research and technique · 2017Article
- 3D printed nerve guidance channels: computer-aided control of geometry, physical cues, biological supplements and gradients.Neural regeneration research · 2016Article
- Approaches to Peripheral Nerve Repair: Generations of Biomaterial Conduits Yielding to Replacing Autologous Nerve Grafts in Craniomaxillofacial Surgery.BioMed research international · 2016Review
- Peripheral Nerve Regeneration by Secretomes of Stem Cells from Human Exfoliated Deciduous Teeth.Stem cells and development · 2015Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
With the aim of forming bioactive guides for peripheral nerve regeneration, silk fibroin was electrospun to obtain aligned nanofibers. These fibers were functionalized by incorporating Nerve Growth Factor (NGF) and Ciliary NeuroTrophic Factor (CNTF) during electrospinning. PC12 cells grown on the fibers confirmed the bioavailability and bioactivity of the NGF, which was not significantly released from the fibers. Primary neurons from rat dorsal root ganglia (DRGs) were grown on the nanofibers and anchored to the fibers and grew in a directional fashion based on the fiber orientation, and as confirmed by growth cone morphology. These biofunctionalized nanofibers led to a 3-fold increase in neurite length at their contact, which was likely due to the NGF. Glial cell growth, alignment and migration were stimulated by the CNTF in the functionalized nanofibers. Organotypic culture of rat fetal DRGs confirmed the complementary effect of both growth factors in multifunctionalized nanofibers, which allowed glial cell migration, alignment and parallel axonal growth in structures resembling the 'bands of Bungner' found in situ. Graftable multi-channel conduits based on biofunctionalized aligned silk nanofibers were developed as an organized 3D scaffold. Our bioactive silk tubes thus represent new options for a biological and biocompatible nerve guidance conduit.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.