ArticleIntravital2014
Finding the bottom and using it: Offsets and sensitivity in the detection of low intensity values in vivo with 2-photon microscopy.
Article in Intravital, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Intravital Microscopy Reveals Unforeseen Biodistribution Within the Liver and Kidney Mechanistically Connected to the Clearance of a Bifunctional Antibody.Drug metabolism and disposition: the biological fate of chemicals · 2023Article
- Albumin uptake and processing by the proximal tubule: physiological, pathological, and therapeutic implications.Physiological reviews · 2022Review
- New insights into proteinuria/albuminuria.Frontiers in physiology · 2022Review
- Intravital Multiphoton Microscopy as a Tool for Studying Renal Physiology, Pathophysiology and Therapeutics.Frontiers in physiology · 2022Review
- Mechanism of how carbamylation reduces albumin binding to FcRn contributing to increased vascular clearance.American journal of physiology. Renal physiology · 2021Article
- Fluorescent Imaging and Microscopy for Dynamic Processes in Rats.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Live-Animal Imaging of Renal Function by Multiphoton Microscopy.Current protocols in cytometry · 2018Article
- Intravital imaging of the kidney.Methods (San Diego, Calif.) · 2017Article
- Intravital multiphoton microscopy as a tool for studying renal physiology and pathophysiology.Methods (San Diego, Calif.) · 2017Review
- Spatiotemporal Rank Filtering Improves Image Quality Compared to Frame Averaging in 2-Photon Laser Scanning Microscopy.PloS one · 2016Article
- Letter to the editor: "Quantifying albumin permeability with multiphoton microscopy: why the difference?".American journal of physiology. Renal physiology · 2014Article
- The proximal tubule and albuminuria: really!Journal of the American Society of Nephrology : JASN · 2014Review
- Albuminuria is controlled primarily by proximal tubules.Nature reviews. Nephrology · 2014Article
- Using 2-photon microscopy to understand albuminuria.Transactions of the American Clinical and Climatological Association · 2014Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Maximizing 2-photon parameters used in acquiring images for quantitative intravital microscopy, especially when high sensitivity is required, remains an open area of investigation. Here we present data on correctly setting the black level of the photomultiplier tube amplifier by adjusting the offset to allow for accurate quantitation of low intensity processes. When the black level is set too high some low intensity pixel values become zero and a nonlinear degradation in sensitivity occurs rendering otherwise quantifiable low intensity values virtually undetectable. Initial studies using a series of increasing offsets for a sequence of concentrations of fluorescent albumin in vitro revealed a loss of sensitivity for higher offsets at lower albumin concentrations. A similar decrease in sensitivity, and therefore the ability to correctly determine the glomerular permeability coefficient of albumin, occurred in vivo at higher offset. Finding the offset that yields accurate and linear data are essential for quantitative analysis when high sensitivity is required.
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Registered trials
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