ArticleJournal of parasitology research2014
Morphological Features and In Vitro Cytopathic Effect of Acanthamoeba griffini Trophozoites Isolated from a Clinical Case.
Article in Journal of parasitology research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- Characterization ofFrontiers in cellular and infection microbiology · 2025Article
- Influence of salt and temperature in the growth of pathogenic free-living amoebae.Frontiers in microbiology · 2024Article
- Induction of Programmed Cell Death inAntioxidants (Basel, Switzerland) · 2023Article
- Taurine, a Component of the Tear Film, Exacerbates the Pathogenic Mechanisms ofPathogens (Basel, Switzerland) · 2023Article
- In Vitro Cytopathogenic Activities ofPathogens (Basel, Switzerland) · 2022Article
- High oxygen concentrations inhibit Acanthamoeba spp.Parasitology research · 2021Article
- Discovery of Amoebicidal Compounds by Combining Computational and Experimental Approaches.Antimicrobial agents and chemotherapy · 2021Article
- Article
- In vitro evaluation of commercial foam Belcils® on Acanthamoeba spp.International journal for parasitology. Drugs and drug resistance · 2020Article
- Ultrastructural, Cytochemical, and Comparative Genomic Evidence of Peroxisomes in Three Genera of Pathogenic Free-Living Amoebae, Including the First Morphological Data for the Presence of This Organelle in Heteroloboseans.Genome biology and evolution · 2020Article
- Extracellular protease profile of Acanthamoeba after prolonged axenic culture and after interaction with MDCK cells.Parasitology research · 2020Article
- Antiamoebic Activities of Indolocarbazole Metabolites Isolated fromMarine drugs · 2019Article
- Ursolic Acid Derivatives as Potential Agents AgainstPathogens (Basel, Switzerland) · 2019Article
- Article
- Evaluation of Oxasqualenoids from the Red AlgaMarine drugs · 2019Article
- Toxic effects of selected proprietary dry eye drops on Acanthamoeba.Scientific reports · 2018Article
- Acanthamoeba of three morphological groups and distinct genotypes exhibit variable and weakly inter-related physiological properties.Parasitology research · 2018Article
- The many lives of nontuberculous mycobacteria.Journal of bacteriology · 2018Article
- Acanthamoeba genotypes T3 and T4 as causative agents of amoebic keratitis in Mexico.Parasitology research · 2016Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Light and transmission electron microscopy observations are reported on the structure and in vitro cytopathic effect of Acanthamoeba griffini trophozoites isolated from a clinical case. Live trophozoites were moderately active with a remarkable pleomorphism which changed from ovoid to quite elongated shapes. When moving, amoebae formed cytoplasmic projections such as wide lamellae and acanthopodia of diverse size and thickness which contain a significant amount of actin. Ultrastructurally, the cytoplasm showed the main organelles found in other free-living amoebae. Coincubation of trophozoites with MDCK cell monolayers resulted in a local damage to target cells after 24 h of interaction, suggesting that the cytopathic effect is contact-dependent. By transmission electron microscopy, amoebae appeared to engulf small portions of the MDCK cells; however, the cells that were not in contact with trophozoites had an unaltered morphology. When epithelial monolayers were incubated with conditioned medium for 24 h, small areas of cell injury were also observed. The phylogenetical analysis as well as the sequencing of the acquired amplified product for the DF3 region of the amoebae isolate confirmed that it belongs to genotype T3, which includes other pathogenic amoebae; besides the activity of two drugs currently used against Acanthamoeba was tested on A. griffini.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.