ArticleProceedings of the National Academy of Sciences of the United States of America2014
A nonpyrrolysine member of the widely distributed trimethylamine methyltransferase family is a glycine betaine methyltransferase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 93 citations in OpenAlex.
- Trimethylamine-N-oxide and its biological variations in vegetarians.European journal of nutrition · 2017Trial
- Nonnative Thiol and Aniline Methylation, Including Enantioselective Dithiol Desymmetrization, by Multicomponent Cobamide-Dependent Methyltransferases Using Unactivated Methyl Donors.Angewandte Chemie (International ed. in English) · 2026Article
- Algal Betaine Triggers Bacterial Hydrogen Peroxide Production that Promotes Algal Demise.Nature communications · 2026Article
- Delineation of the active site of MtgB, a cobalamin-dependent glycine betaine methyltransferase.The Journal of biological chemistry · 2026Article
- Methanogenesis from tetramethylammonium and choline inFrontiers in microbiology · 2026Article
- Metagenome-assembled genomes reveal microbial signatures and metabolic pathways linked to coronary artery disease.mSystems · 2025Article
- Methionine synthesis and glycine betaine demethylation are intricately intertwined in cosmopolitan marine bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Microbial dark matter spearheading the biogeochemical cycle in the Solar Lake of Taba, Egypt.Current research in microbial sciences · 2025Article
- Article
- Asgard archaea modulate potential methanogenesis substrates in wetland soil.Nature communications · 2024Article
- Glycine betaine metabolism is enabled inApplied and environmental microbiology · 2024Article
- Review
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- Characterizing complete mitochondrial genome of Aquilegia amurensis and its evolutionary implications.BMC plant biology · 2024Article
- Methylotrophy in the Mire: direct and indirect routes for methane production in thawing permafrost.mSystems · 2024Article
- A diversified, widespread microbial gene cluster encodes homologs of methyltransferases involved in methanogenesis.bioRxiv : the preprint server for biology · 2023Article
- WidespreadmLife · 2023Article
- Human Gut Microbiota and Drug Metabolism.Microbial ecology · 2023Review
- Methyl-Based Methanogenesis: an Ecological and Genomic Review.Microbiology and molecular biology reviews : MMBR · 2023Review
- Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
COG5598 comprises a large number of proteins related to MttB, the trimethylamine:corrinoid methyltransferase. MttB has a genetically encoded pyrrolysine residue proposed essential for catalysis. MttB is the only known trimethylamine methyltransferase, yet the great majority of members of COG5598 lack pyrrolysine, leaving the activity of these proteins an open question. Here, we describe the function of one of the nonpyrrolysine members of this large protein family. Three nonpyrrolysine MttB homologs are encoded in Desulfitobacterium hafniense, a Gram-positive strict anaerobe present in both the environment and human intestine. D. hafniense was found capable of growth on glycine betaine with electron acceptors such as nitrate or fumarate, producing dimethylglycine and CO2 as products. Examination of the genome revealed genes for tetrahydrofolate-linked oxidation of a methyl group originating from a methylated corrinoid protein, but no obvious means to carry out corrinoid methylation with glycine betaine. DSY3156, encoding one of the nonpyrrolysine MttB homologs, was up-regulated during growth on glycine betaine. The recombinant DSY3156 protein converts glycine betaine and cob(I)alamin to dimethylglycine and methylcobalamin. To our knowledge, DSY3156 is the first glycine betaine:corrinoid methyltransferase described, and a designation of MtgB is proposed. In addition, DSY3157, an adjacently encoded protein, was shown to be a methylcobalamin:tetrahydrofolate methyltransferase and is designated MtgA. Homologs of MtgB are widely distributed, especially in marine bacterioplankton and nitrogen-fixing plant symbionts. They are also found in multiple members of the human microbiome, and may play a beneficial role in trimethylamine homeostasis, which in recent years has been directly tied to human cardiovascular health.
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