ArticleProceedings of the National Academy of Sciences of the United States of America2014
The protein Compromised Hydrolysis of Triacylglycerols 7 (CHT7) acts as a repressor of cellular quiescence in Chlamydomonas.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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42 citing papers in PubMed.
- Article
- Regulatory network rewiring drives strain-specific lipid accumulation response in Chlorella sorokiniana under nutrient starvation.The Plant journal : for cell and molecular biology · 2026Article
- Identification and Overexpression of Endogenous Transcription Factors to Enhance Lipid Accumulation in the Commercially Relevant SpeciesbioRxiv : the preprint server for biology · 2025Article
- Co-option of plant gene regulatory network in nutrient responses during terrestrialization.Nature plants · 2024Article
- α/β hydrolase domain-containing protein 1 acts as a lysolipid lipase and is involved in lipid droplet formation.National science review · 2024Article
- Engineering Fatty Acid Biosynthesis in Microalgae: Recent Progress and Perspectives.Marine drugs · 2024Review
- Harnessing genetic engineering to drive economic bioproduct production in algae.Frontiers in bioengineering and biotechnology · 2024Review
- Chlamydomonas CHT7 is involved in repressing DNA replication and mitotic genes during synchronous growth.G3 (Bethesda, Md.) · 2022Article
- Large fluxes of fatty acids from membranes to triacylglycerol and back during N-deprivation and recovery in Chlamydomonas.Plant physiology · 2021Article
- Channeling of Carbon Flux Towards Carotenogenesis inFrontiers in microbiology · 2021Article
- Modulation of CHT7 Complexes during Light/Dark- and Nitrogen-Mediated Life Cycle Transitions of Chlamydomonas.Plant physiology · 2020Article
- Identification of Polyunsaturated Fatty Acids Synthesis Pathways in the Toxic DinophyteBiomolecules · 2020Article
- When to Sleep? CHT7 Is Critical for Nutrient-Dependent Quiescence in Chlamydomonas.The Plant cell · 2020Article
- Chlamydomonas CHT7 Is Required for an Effective Quiescent State by Regulating Nutrient-Responsive Cell Cycle Gene Expression.The Plant cell · 2020Article
- The MicroalgaPlant physiology · 2020Article
- CrABCA2 Facilitates Triacylglycerol Accumulation inMolecules and cells · 2020Article
- Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application.Frontiers in bioengineering and biotechnology · 2020Review
- Lipidome Remodeling and Autophagic Respose in the Arachidonic-Acid-Rich MicroalgaFrontiers in plant science · 2020Article
- Lipid remodeling regulator 1 (LRL1) is differently involved in the phosphorus-depletion response from PSR1 in Chlamydomonas reinhardtii.The Plant journal : for cell and molecular biology · 2019Article
- Article
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Abstract
Microalgae are prolific photosynthetic organisms that have the potential to sustainably produce high-value chemical feedstocks. However, an industry based on microalgal biomass still is faced with challenges. For example, microalgae tend to accumulate valuable compounds, such as triacylglycerols, only under stress conditions that limit growth. To investigate the fundamental mechanisms at the base of this conundrum--the inverse relationship between biomass production and storage compound accumulation-we applied a combination of cell biological and genetic approaches. Conceptually, nutrient deprivation, which commonly is used to induce the accumulation of triacylglycerol in microalgae, leads to a state of cellular quiescence defined by a halt of cell divisions that is reversible upon nutrient resupply. To identify factors that govern cellular quiescence, we screened for mutants of the model alga Chlamydomonas reinhardtii that, in contrast to wild-type cells placed under conditions of nitrogen deprivation, were unable to degrade triacylglycerols following nitrogen resupply. One of the mutants described here in detail, compromised hydrolysis of triacylglycerols 7 (cht7), was severely impaired in regrowth following removal of different conditions inducing cellular quiescence. The mutant carries a deletion affecting four genes, only one of which rescued the quiescence phenotype when reintroduced. It encodes a protein with similarity to mammalian and plant DNA binding proteins. Comparison of transcriptomes indicated a partial derepression of quiescence-related transcriptional programs in the mutant under conditions favorable to growth. Thus, CHT7 likely is a repressor of cellular quiescence and provides a possible target for the engineering of high-biomass/high-triacylglycerol microalgae.
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