ArticleProceedings of the National Academy of Sciences of the United States of America2014
Interplay of Mg2+, ADP, and ATP in the cytosol and mitochondria: unravelling the role of Mg2+ in cell respiration.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 115 papers.
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115 citing papers in PubMed, 206 citations in OpenAlex.
- Magnesium and nerve injury: Mechanisms and applications.Neural regeneration research · 2026Article
- Magnesium and Diabetes: A Review of Recent Studies on the Efficacy of Supplementation with Mg in Humans Suffering from This Chronic Metabolic Disease.International journal of molecular sciences · 2026Review
- Distinct Mg²⁺ responses and species-specific transcriptional regulation of the magnesium transporter MGR2 in Saccharum officinarum and Saccharum spontaneum.BMC plant biology · 2026Article
- Aggregation of Phosphates Enhances Enzyme Activity in Aqueous Solution.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- How Plants May Maintain Protein Homeostasis Under Rising Atmospheric COPlant, cell & environment · 2026Review
- The oncogenic CCDC6-RET fusion protein is a dual ATP- and ADP-dependent kinase.Nature communications · 2026Article
- Characterization of the enzymatic activity of the rhythmic human NADP(H) phosphatase, Nocturnin.Archives of biochemistry and biophysics · 2026Article
- Magnesium: An overlooked signalling ion in plant physiology and circadian regulation.Quantitative plant biology · 2026Review
- Comparative genomics revealed the gene evolution and functional divergence ofFrontiers in plant science · 2026Article
- Chloroplast-mitochondria synergy modulates responses to iron limitation in twoISME communications · 2026Article
- Micro- and Nanoplastics Act as Metal Carriers with the Potential to Alter Human Gene Expression Patterns-The Inferences from Bioinformatic Online Tools.Biomolecules · 2025Review
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- ATP allosterically regulates an acyl-CoA oxidase.Nature communications · 2025Article
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- Cytochrome c oxidase inactivation in Physcomitrium patens reveals that respiration coordinates plant metabolism.The Plant cell · 2025Article
- Five unaddressed questions about cytokinin biosynthesis.Journal of experimental botany · 2025Review
- Linear or Cyclic? Theoretical Investigation of Astrophysically Relevant Magnesium-Bearing MgCJournal of computational chemistry · 2025Article
- Low magnetic fields stimulate cardiac mitochondrial bioenergetics with a bell-shaped response: Possibly via a radical pair mechanism.Computational and structural biotechnology journal · 2025Article
- De novo designed YK peptides forming reversible amyloid for synthetic protein condensates in mammalian cells.Nature communications · 2024Article
- Influence of Magnesium Ion Binding on the Adenosine Diphosphate Structure and Dynamics, Investigated byThe journal of physical chemistry. B · 2024Article
55 more citing papers are in PubMed but not listed here.
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4 authors at 4 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
In animal and plant cells, the ATP/ADP ratio and/or energy charge are generally considered key parameters regulating metabolism and respiration. The major alternative issue of whether the cytosolic and mitochondrial concentrations of ADP and ATP directly mediate cell respiration remains unclear, however. In addition, because only free nucleotides are exchanged by the mitochondrial ADP/ATP carrier, whereas MgADP is the substrate of ATP synthase (EC 3.6.3.14), the cytosolic and mitochondrial Mg(2+) concentrations must be considered as well. Here we developed in vivo/in vitro techniques using (31)P-NMR spectroscopy to simultaneously measure these key components in subcellular compartments. We show that heterotrophic sycamore (Acer pseudoplatanus L.) cells incubated in various nutrient media contain low, stable cytosolic ADP and Mg(2+) concentrations, unlike ATP. ADP is mainly free in the cytosol, but complexed by Mg(2+) in the mitochondrial matrix, where [Mg(2+)] is tenfold higher. In contrast, owing to a much higher affinity for Mg(2+), ATP is mostly complexed by Mg(2+) in both compartments. Mg(2+) starvation used to alter cytosolic and mitochondrial [Mg(2+)] reversibly increases free nucleotide concentration in the cytosol and matrix, enhances ADP at the expense of ATP, decreases coupled respiration, and stops cell growth. We conclude that the cytosolic ADP concentration, and not ATP, ATP/ADP ratio, or energy charge, controls the respiration of plant cells. The Mg(2+) concentration, remarkably constant and low in the cytosol and tenfold higher in the matrix, mediates ADP/ATP exchange between the cytosol and matrix, [MgADP]-dependent mitochondrial ATP synthase activity, and cytosolic free ADP homeostasis.
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