Evidence map›Paper›PMID 25312645›Full record

ArticleMolecular and cellular biology2014

An absence of nuclear lamins in keratinocytes leads to ichthyosis, defective epidermal barrier function, and intrusion of nuclear membranes and endoplasmic reticulum into the nuclear chromatin.

Hea-Jin Jung, Angelica Tatar, Yiping Tu, Chika Nobumori, Shao H Yang, Chris N Goulbourne, Harald Herrmann, Loren G Fong, Stephen G Young

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and cellular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
1.1field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 26 citations in OpenAlex.

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  4. Increased expression of LAP2β eliminates nuclear membrane ruptures in nuclear lamin-deficient neurons and fibroblasts.Proceedings of the National Academy of Sciences of the United States of America · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Hea-Jin JungMolecular Biology Institute, University of California, Los Angeles, California, USA.
Angelica TatarDepartment of Medicine, University of California, Los Angeles, California, USA.
Yiping TuDepartment of Medicine, University of California, Los Angeles, California, USA.
Chika NobumoriDepartment of Medicine, University of California, Los Angeles, California, USA.
Shao H YangDepartment of Medicine, University of California, Los Angeles, California, USA.
Chris N GoulbourneDepartment of Medicine, University of California, Los Angeles, California, USA.
Harald HerrmannGerman Cancer Research Center, Heidelberg, Germany.
Loren G FongDepartment of Medicine, University of California, Los Angeles, California, USA lfong@mednet.ucla.edu sgyoung@mednet.ucla.edu.
Stephen G YoungMolecular Biology Institute, University of California, Los Angeles, California, USA Department of Medicine, University of California, Los Angeles, California, USA Department of Human Genetics, University of California, Los Angeles, California, USA lfong@mednet.ucla.edu sgyoung@mednet.ucla.edu.
University of California, Los Angeles · USGerman Cancer Research Center · DE

Funding

New Therapeutic Approaches for Hutchinson-Gilford Progeria SyndromeR01HL086683 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI FONG, LOREN GI · 2007 to 2011
$2.2M
CaaX Protein Processing and Human DiseaseR01AG035626 · NIA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI YOUNG, STEPHEN G. · 2009 to 2013
$2.0M
Protein Prenylation and ProgeriaR01HL089781 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI FONG, LOREN GI · 2009 to 2013
$1.9M
NHLBI NIH HHS HL089781NHLBI NIH HHS HL86683NHLBI NIH HHS R01 HL086683NHLBI NIH HHS R01 HL089781NIA NIH HHS R01 AG035626
6 · The paper itself

Abstract

B-type lamins (lamins B1 and B2) have been considered to be essential for many crucial functions in the cell nucleus (e.g., DNA replication and mitotic spindle formation). However, this view has been challenged by the observation that an absence of both B-type lamins in keratinocytes had no effect on cell proliferation or the development of skin and hair. The latter findings raised the possibility that the functions of B-type lamins are subserved by lamins A and C. To explore that idea, we created mice lacking all nuclear lamins in keratinocytes. Those mice developed ichthyosis and a skin barrier defect, which led to death from dehydration within a few days after birth. Microscopy of nuclear-lamin-deficient skin revealed hyperkeratosis and a disordered stratum corneum with an accumulation of neutral lipid droplets; however, BrdU incorporation into keratinocytes was normal. Skin grafting experiments confirmed the stratum corneum abnormalities and normal BrdU uptake. Interestingly, the absence of nuclear lamins in keratinocytes resulted in an interspersion of nuclear/endoplasmic reticulum membranes with the chromatin. Thus, a key function of the nuclear lamina is to serve as a "fence" and prevent the incursion of cytoplasmic organelles into the nuclear chromatin.

Indexed as

AnimalsAnimals, NewbornBromodeoxyuridineCell ProliferationChromatinDisease Models, AnimalEndoplasmic ReticulumFemaleIchthyosisKeratinocytesLaminsMiceMice, NudeSkinBromodeoxyuridineChromatinLamins

Identifiers

PMID25312645
PMCPMC4248738
OpenAlexW2125661978

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.