ArticleExperimental animals2015
Identification of causative pathogens in mouse eyes with bacterial keratitis by sequence analysis of 16S rDNA libraries.
Article in Experimental animals, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 8 citations in OpenAlex.
- Alterations of the bacterial ocular surface microbiome are found in both eyes of horses with unilateral ulcerative keratitis.PloS one · 2023Article
- Challenges and insights in the exploration of the low abundance human ocular surface microbiome.Frontiers in cellular and infection microbiology · 2023Article
- Poloxamer and Chitosan-BasedTurkish journal of pharmaceutical sciences · 2022Article
- Isolation of Streptococcus cuniculi from corneal lesion in laboratory-raised mice.The Journal of veterinary medical science · 2021Article
- The anti-inflammatory effects of asiatic acid in lipopolysaccharide-stimulated human corneal epithelial cells.International journal of ophthalmology · 2017Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The clone library method using PCR amplification of the 16S ribosomal RNA (rRNA) gene was used to identify pathogens from corneal scrapings of C57BL/6-corneal opacity (B6-Co) mice with bacterial keratitis. All 10 samples from the eyes with bacterial keratitis showed positive PCR results. All 10 samples from the normal cornea showed negative PCR results. In all 10 PCR-positive samples, the predominant and second most predominant species accounted for 20.9 to 40.6% and 14.7 to 26.1%, respectively, of each clone library. The predominant species were Staphylococcus lentus, Pseudomonas aeruginosa, and Staphylococcus epidermidis. The microbiota analysis detected a diverse group of microbiota in the eyes of B6-Co mice with bacterial keratitis and showed that the causative pathogens could be determined based on percentages of bacterial species in the clone libraries. The bacterial species detected in this study were mostly in accordance with results of studies on clinical bacterial keratitis in human eyes. Based on the results of our previous studies and this study, the B6-Co mouse should be considered a favorable model for studying bacterial keratitis.
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