ArticleBiotechnology and bioengineering2015
Metabolomic and (13)C-metabolic flux analysis of a xylose-consuming Saccharomyces cerevisiae strain expressing xylose isomerase.
Article in Biotechnology and bioengineering, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Engineering mixed sugar metabolic channels inSynthetic and systems biotechnology · 2026Article
- Article
- Modular deregulation of central carbon metabolism for efficient xylose utilization in Saccharomyces cerevisiae.Nature communications · 2025Article
- Sustainable Food Waste Management in Anaerobic Digesters: Prediction of the Organic Load Impact by Metagenome-Scale Metabolic Modeling.Environmental science & technology · 2025Article
- Chemobiosis reveals tardigrade tun formation is dependent on reversible cysteine oxidation.PloS one · 2024Article
- Construction of a xylose metabolic pathway in Trichosporonoides oedocephalis ATCC 16958 for the production of erythritol and xylitol.Biotechnology letters · 2023Article
- High throughputMicrobial cell factories · 2023Article
- D-xylose accelerated death of pentose metabolizing Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2023Article
- mfapy: An open-source Python package forMetabolic engineering communications · 2021Article
- D-Xylose Sensing inInternational journal of molecular sciences · 2021Review
- Structure-based directed evolution improvesBiotechnology for biofuels · 2020Article
- Rewiring carbon metabolism in yeast for high level production of aromatic chemicals.Nature communications · 2019Article
- Metabolic fluxes-oriented control of bioreactors: a novel approach to tune micro-aeration and substrate feeding in fermentations.Microbial cell factories · 2019Article
- Metabolic flux analysis for metabolome data validation of naturally xylose-fermenting yeasts.BMC biotechnology · 2019Article
- Heterologous phosphoketolase expression redirects flux towards acetate, perturbs sugar phosphate pools and increases respiratory demand in Saccharomyces cerevisiae.Microbial cell factories · 2019Article
- Pentose Metabolism in Saccharomyces cerevisiae: The Need to Engineer Global Regulatory Systems.Biotechnology journal · 2019Review
- Multi-Omics Analysis of Fatty Alcohol Production in Engineered YeastsFrontiers in genetics · 2019Article
- A comprehensive evaluation of constraining amino acid biosynthesis in compartmented models for metabolic flux analysis.Metabolic engineering communications · 2017Article
- Quantitative metabolomics of a xylose-utilizing Saccharomyces cerevisiae strain expressing the Bacteroides thetaiotaomicron xylose isomerase on glucose and xylose.Journal of industrial microbiology & biotechnology · 2017Article
- Elucidating dynamic metabolic physiology through network integration of quantitative time-course metabolomics.Scientific reports · 2017Article
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2 authors.
Funding
Abstract
Over the past two decades, significant progress has been made in the engineering of xylose-consuming Saccharomyces cerevisiae strains for production of lignocellulosic biofuels. However, the ethanol productivities achieved on xylose are still significantly lower than those observed on glucose for reasons that are not well understood. We have undertaken an analysis of central carbon metabolite pool sizes and metabolic fluxes on glucose and on xylose under aerobic and anaerobic conditions in a strain capable of rapid xylose assimilation via xylose isomerase in order to investigate factors that may limit the rate of xylose fermentation. We find that during xylose utilization the flux through the non-oxidative Pentose Phosphate Pathway (PPP) is high but the flux through the oxidative PPP is low, highlighting an advantage of the strain employed in this study. Furthermore, xylose fails to elicit the full carbon catabolite repression response that is characteristic of glucose fermentation in S. cerevisiae. We present indirect evidence that the incomplete activation of the fermentation program on xylose results in a bottleneck in lower glycolysis, leading to inefficient re-oxidation of NADH produced in glycolysis.
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