Evidence map›Paper›PMID 25311696›Full record

ArticleThe American journal of tropical medicine and hygiene2014

Comparison of point-of-care tests for the rapid diagnosis of visceral leishmaniasis in East African patients.

Asrat Bezuneh, Maowia Mukhtar, Asim Abdoun, Tedla Teferi, Yegnasew Takele, Ermias Diro, Asfaw Jemaneh, Welelta Shiferaw, Hirut Wondimu, Ajay Bhatia and 5 more

Open access · bronzeAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in The American journal of tropical medicine and hygiene, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed, 3 pooled it
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 3 syntheses or guidelines pooled it, 47 citations in OpenAlex.

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  6. The potential ofMicrobiology spectrum · 2025
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  15. A critical review of the applicability of serological screening for Leishmaniasis in blood banks in Brazil.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 2 institutions in 1 country.

Asrat BezunehDepartment of Microbiology, Immunology and Parasitology, Addis Ababa University, Addis Ababa, Ethiopia; Institute of Endemic Disease, University of Khartoum, Khartoum, Sudan; Leishmaniasis Research and Treatment Centre, Arba-Minch Hospital, Ethiopia; Leishmaniasis Research and Treatment Centre, University of Gondar, Ethiopia; Infectious Disease Research Institute, Seattle, Washington.
Maowia MukhtarDepartment of Microbiology, Immunology and Parasitology, Addis Ababa University, Addis Ababa, Ethiopia; Institute of Endemic Disease, University of Khartoum, Khartoum, Sudan; Leishmaniasis Research and Treatment Centre, Arba-Minch Hospital, Ethiopia; Leishmaniasis Research and Treatment Centre, University of Gondar, Ethiopia; Infectious Disease Research Institute, Seattle, Washington.
Asim AbdounDepartment of Microbiology, Immunology and Parasitology, Addis Ababa University, Addis Ababa, Ethiopia; Institute of Endemic Disease, University of Khartoum, Khartoum, Sudan; Leishmaniasis Research and Treatment Centre, Arba-Minch Hospital, Ethiopia; Leishmaniasis Research and Treatment Centre, University of Gondar, Ethiopia; Infectious Disease Research Institute, Seattle, Washington.
Tedla TeferiDepartment of Microbiology, Immunology and Parasitology, Addis Ababa University, Addis Ababa, Ethiopia; Institute of Endemic Disease, University of Khartoum, Khartoum, Sudan; Leishmaniasis Research and Treatment Centre, Arba-Minch Hospital, Ethiopia; Leishmaniasis Research and Treatment Centre, University of Gondar, Ethiopia; Infectious Disease Research Institute, Seattle, Washington.
Yegnasew TakeleDepartment of Microbiology, Immunology and Parasitology, Addis Ababa University, Addis Ababa, Ethiopia; Institute of Endemic Disease, University of Khartoum, Khartoum, Sudan; Leishmaniasis Research and Treatment Centre, Arba-Minch Hospital, Ethiopia; Leishmaniasis Research and Treatment Centre, University of Gondar, Ethiopia; Infectious Disease Research Institute, Seattle, Washington.
Ermias DiroDepartment of Microbiology, Immunology and Parasitology, Addis Ababa University, Addis Ababa, Ethiopia; Institute of Endemic Disease, University of Khartoum, Khartoum, Sudan; Leishmaniasis Research and Treatment Centre, Arba-Minch Hospital, Ethiopia; Leishmaniasis Research and Treatment Centre, University of Gondar, Ethiopia; Infectious Disease Research Institute, Seattle, Washington.
Asfaw JemanehDepartment of Microbiology, Immunology and Parasitology, Addis Ababa University, Addis Ababa, Ethiopia; Institute of Endemic Disease, University of Khartoum, Khartoum, Sudan; Leishmaniasis Research and Treatment Centre, Arba-Minch Hospital, Ethiopia; Leishmaniasis Research and Treatment Centre, University of Gondar, Ethiopia; Infectious Disease Research Institute, Seattle, Washington.
Welelta ShiferawDepartment of Microbiology, Immunology and Parasitology, Addis Ababa University, Addis Ababa, Ethiopia; Institute of Endemic Disease, University of Khartoum, Khartoum, Sudan; Leishmaniasis Research and Treatment Centre, Arba-Minch Hospital, Ethiopia; Leishmaniasis Research and Treatment Centre, University of Gondar, Ethiopia; Infectious Disease Research Institute, Seattle, Washington.
Hirut WondimuDepartment of Microbiology, Immunology and Parasitology, Addis Ababa University, Addis Ababa, Ethiopia; Institute of Endemic Disease, University of Khartoum, Khartoum, Sudan; Leishmaniasis Research and Treatment Centre, Arba-Minch Hospital, Ethiopia; Leishmaniasis Research and Treatment Centre, University of Gondar, Ethiopia; Infectious Disease Research Institute, Seattle, Washington.
Ajay BhatiaDepartment of Microbiology, Immunology and Parasitology, Addis Ababa University, Addis Ababa, Ethiopia; Institute of Endemic Disease, University of Khartoum, Khartoum, Sudan; Leishmaniasis Research and Treatment Centre, Arba-Minch Hospital, Ethiopia; Leishmaniasis Research and Treatment Centre, University of Gondar, Ethiopia; Infectious Disease Research Institute, Seattle, Washington.
Randall F HowardDepartment of Microbiology, Immunology and Parasitology, Addis Ababa University, Addis Ababa, Ethiopia; Institute of Endemic Disease, University of Khartoum, Khartoum, Sudan; Leishmaniasis Research and Treatment Centre, Arba-Minch Hospital, Ethiopia; Leishmaniasis Research and Treatment Centre, University of Gondar, Ethiopia; Infectious Disease Research Institute, Seattle, Washington.
Hashim GhalibDepartment of Microbiology, Immunology and Parasitology, Addis Ababa University, Addis Ababa, Ethiopia; Institute of Endemic Disease, University of Khartoum, Khartoum, Sudan; Leishmaniasis Research and Treatment Centre, Arba-Minch Hospital, Ethiopia; Leishmaniasis Research and Treatment Centre, University of Gondar, Ethiopia; Infectious Disease Research Institute, Seattle, Washington.
Gregory C IretonDepartment of Microbiology, Immunology and Parasitology, Addis Ababa University, Addis Ababa, Ethiopia; Institute of Endemic Disease, University of Khartoum, Khartoum, Sudan; Leishmaniasis Research and Treatment Centre, Arba-Minch Hospital, Ethiopia; Leishmaniasis Research and Treatment Centre, University of Gondar, Ethiopia; Infectious Disease Research Institute, Seattle, Washington.
Asrat HailuDepartment of Microbiology, Immunology and Parasitology, Addis Ababa University, Addis Ababa, Ethiopia; Institute of Endemic Disease, University of Khartoum, Khartoum, Sudan; Leishmaniasis Research and Treatment Centre, Arba-Minch Hospital, Ethiopia; Leishmaniasis Research and Treatment Centre, University of Gondar, Ethiopia; Infectious Disease Research Institute, Seattle, Washington.
Steven G ReedDepartment of Microbiology, Immunology and Parasitology, Addis Ababa University, Addis Ababa, Ethiopia; Institute of Endemic Disease, University of Khartoum, Khartoum, Sudan; Leishmaniasis Research and Treatment Centre, Arba-Minch Hospital, Ethiopia; Leishmaniasis Research and Treatment Centre, University of Gondar, Ethiopia; Infectious Disease Research Institute, Seattle, Washington steven.reed@idri.org.
Infectious Disease Research Institute · USSeattle University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The development of rK39-based rapid diagnostic tests (RDTs) has greatly aided the diagnosis of visceral leishmaniasis, especially in the Indian subcontinent and Brazil, by offering high sensitivity and specificity. However, these tests have been less sensitive and less specific in sub-Saharan Africa. To improve upon the performance of rK39 in Africa, we engineered the fusion molecule rK28, which retained some of the rK39 repeats and combined them with repeat sequences from two additional Leishmania genes. This polyprotein was used in the development of several prototype RDTs by different commercial manufacturers with the goal of assessing relative performance in inexpensive formats. Here, we report field studies showing that the rK28 antigen could be readily adapted to a variety of RDT formats to achieve high sensitivity, generally > 90%, and adequate specificity to aid in the diagnosis of human visceral leishmaniasis in East Africa, Asia, and South America.

Indexed as

Point-of-Care SystemsAfrica, EasternHumansLeishmaniasis, VisceralSensitivity and Specificity

Identifiers

PMID25311696
PMCPMC4257631
OpenAlexW2050561021

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.