ArticleNature communications2014
Highly modular bow-tie gene circuits with programmable dynamic behaviour.
Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Non-negative connectivity causes bow-tie architecture in neural circuits.Frontiers in neural circuits · 2025Article
- Orthogonal LoxPsym sites allow multiplexed site-specific recombination in prokaryotic and eukaryotic hosts.Nature communications · 2024Article
- Bow-tie architectures in biological and artificial neural networks: Implications for network evolution and assay design.iScience · 2023Article
- Molecular recording: transcriptional data collection into the genome.Current opinion in biotechnology · 2023Review
- Synthetic gene circuits for cell state detection and protein tuning in human pluripotent stem cells.Molecular systems biology · 2022Article
- Neuroinflammation Following Traumatic Brain Injury: Take It Seriously or Not.Frontiers in immunology · 2022Review
- Context-aware synthetic biology by controller design: Engineering the mammalian cell.Cell systems · 2021Review
- Design of Multipartite Transcription Factors for Multiplexed Logic Genome Integration Control in Mammalian Cells.ACS synthetic biology · 2020Article
- Precise determination of input-output mapping for multimodal gene circuits using data from transient transfection.PLoS computational biology · 2020Article
- Programmable Synthetic Protein Circuits for the Identification and Suppression of Hepatocellular Carcinoma.Molecular therapy oncolytics · 2020Article
- Synthetic control systems for high performance gene expression in mammalian cells.Nucleic acids research · 2018Article
- Stem cell bioengineering: building from stem cell biology.Nature reviews. Genetics · 2018Review
- Designing cell function: assembly of synthetic gene circuits for cell biology applications.Nature reviews. Molecular cell biology · 2018Review
- Genetic programs can be compressed and autonomously decompressed in live cells.Nature nanotechnology · 2018Article
- Model-guided combinatorial optimization of complex synthetic gene networks.Molecular systems biology · 2016Article
- Synthetic Biology Platform for Sensing and Integrating Endogenous Transcriptional Inputs in Mammalian Cells.Cell reports · 2016Article
- Engineering Gene Circuits for Mammalian Cell-Based Applications.Cold Spring Harbor perspectives in biology · 2016Review
- Precision multidimensional assay for high-throughput microRNA drug discovery.Nature communications · 2016Article
- Rationally Designed MicroRNA-Based Genetic Classifiers Target Specific Neurons in the Brain.ACS synthetic biology · 2015Article
- Digital switching in a biosensor circuit via programmable timing of gene availability.Nature chemical biology · 2014Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Synthetic gene circuits often require extensive mutual optimization of their components for successful operation, while modular and programmable design platforms are rare. A possible solution lies in the 'bow-tie' architecture, which stipulates a focal component-a 'knot'-uncoupling circuits' inputs and outputs, simplifying component swapping, and introducing additional layer of control. Here we construct, in cultured human cells, synthetic bow-tie circuits that transduce microRNA inputs into protein outputs with independently programmable logical and dynamic behaviour. The latter is adjusted via two different knot configurations: a transcriptional activator causing the outputs to track input changes reversibly, and a recombinase-based cascade, converting transient inputs into permanent actuation. We characterize the circuits in HEK293 cells, confirming their modularity and scalability, and validate them using endogenous microRNA inputs in additional cell lines. This platform can be used for biotechnological and biomedical applications in vitro, in vivo and potentially in human therapy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.