ArticleThe Journal of reproduction and development2015
Prolonging hypothermic storage (4 C) of bovine embryos with fish antifreeze protein.
Article in The Journal of reproduction and development, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- Development of an oscillating magnetic field-assisted supercooling system for bovine embryo preservation.Journal of animal science and technology · 2026Article
- Role of Glutathione in Alleviating Chilling Injury in Bovine Blastocysts: Mitochondrial Restoration and Apoptosis Inhibition.Antioxidants (Basel, Switzerland) · 2026Article
- Research progress on novel bionic cryoprotectants in gamete and embryo cryopreservation.Frontiers in veterinary science · 2026Review
- The Study of Ice-Binding Protein Oligomeric Complexes.International journal of molecular sciences · 2025Article
- Reduced HSP70 expression under mild hypothermia is involved in bovine embryo growth suppression.The Journal of reproduction and development · 2025Article
- Application of Nanoparticles and Melatonin for Cryopreservation of Gametes and Embryos.Current issues in molecular biology · 2022Review
- Toxicity profiles and protective effects of antifreeze proteins from insect in mammalian models.Toxicology letters · 2022Article
- Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos.International journal of molecular sciences · 2022Article
- Intracellular and Extracellular Antifreeze Protein Significantly Improves Mammalian Cell Cryopreservation.Biomolecules · 2022Article
- Structural diversity of marine anti-freezing proteins, properties and potential applications: a review.Bioresources and bioprocessing · 2022Review
- Subzero Nonfreezing Hypothermia with Insect Antifreeze Protein Dramatically Improves Survival Rate of Mammalian Cells.International journal of molecular sciences · 2021Article
- A brief review of applications of antifreeze proteins in cryopreservation and metabolic genetic engineering.3 Biotech · 2019Review
- Review
- From ice-binding proteins to bio-inspired antifreeze materials.Soft matter · 2017Review
- Marine Antifreeze Proteins: Structure, Function, and Application to Cryopreservation as a Potential Cryoprotectant.Marine drugs · 2017Review
- Microfluidic Cold-Finger Device for the Investigation of Ice-Binding Proteins.Biophysical journal · 2016Article
- Advances in cryopreservation of organs.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Embryos obtained via superovulation are necessary for mammalian artificial reproduction, and viability is a key determinant of success. Nonfreezing storage at 4 C is possible, but currently used storage solutions can maintain embryo viability for only 24-48 h. Here we found that 10 mg/ml antifreeze protein (AFP) dissolved in culture medium 199 with 20% (v/v) fetal bovine serum and 25 mM HEPES could keep bovine embryos alive for 10 days at 4 C. We used a recombinant AFP isolated from the notched-fin eelpout (Zoarces elongatus Kner). Photomicroscopy indicated that the AFP-embryo interaction was enhanced at 37 C. Embryos pre-warmed with the AFP solution at 37 C for 60 min maintained high viability, whereas those that were not pre-warmed could live no longer than 7 days. Thus, short-term storage of bovine embryos was achieved by a combination of AFP-containing medium and controlled pre-warming.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.