ArticleBMC genomics2014
Large proportion of genes in one cryptic WO prophage genome are actively and sex-specifically transcribed in a fig wasp species.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 9 citations in OpenAlex.
- Biochemical characterisation and production kinetics of high molecular-weight (HMW) putative antibacterial proteins of insect pathogenic Brevibacillus laterosporus isolates.BMC microbiology · 2024Article
- Prophages encoding human immune evasion cluster genes are enriched inMicrobial genomics · 2021Article
- Distribution and Evolution of the Bacteriophage WO and Its Antagonism WithFrontiers in microbiology · 2020Article
- Bacteriophage WO Can Mediate Horizontal Gene Transfer in EndosymbioticFrontiers in microbiology · 2016Article
- Novel Moraxella catarrhalis prophages display hyperconserved non-structural genes despite their genomic diversity.BMC genomics · 2015Article
- Dynamics of Wolbachia pipientis Gene Expression Across the Drosophila melanogaster Life Cycle.G3 (Bethesda, Md.) · 2015Article
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Authors and funding
5 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCryptic prophages are genetically defective in their induction and propagation, and are simply regarded as genetic remnants. There are several putative cryptic WO prophages in the sequenced Wolbachia genomes. Whether they are lytic is unclear and their functions are poorly understood. Only three open reading frames (ORFs) in cryptic WO prophages have been reported to be actively transcribed.
resultsIn this study, we comprehensively examined the transcription of the only cryptic WO prophage (WOSol) in a Wolbachia strain that infects a fig wasp, Ceratosolen solmsi (Agaonidae, Chalcidoidea). By analyzing the transcriptions of all the ORFs of WOSol in both sexes of C. solmsi, using qualitative and quantitative methods, we demonstrated that i) a high percentage of ORFs are actively transcribed (59%, 17/29); ii) the expression of these ORFs is highly sex-specific, with a strong male bias (three in females and 15 in males); iii) an ank (ankyrin-domain-containing) gene actively transcribed in both wasp sexes is more highly expressed in males.
conclusionsA large proportion of the genes in the cryptic WO prophage WOSol are expressed, which overturns the concept that cryptic prophages are simply genetically defective. The highly sex-specific expression patterns of these genes in the host suggest that they play important roles in Wolbachia biology and its reproductive manipulation of its insect host, particularly through the males.
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