Evidence map›Paper›PMID 25310989›Full record

ArticleScientific reports2014

Combined biophysical and soluble factor modulation induces cardiomyocyte differentiation from human muscle derived stem cells.

Jason Tchao, Lu Han, Bo Lin, Lei Yang, Kimimasa Tobita

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Electrically Driven Microengineered Bioinspired Soft Robots.Advanced materials (Deerfield Beach, Fla.) · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jason TchaoDepartment of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.
Lu HanDevelopmental Biology, University of Pittsburgh, Pittsburgh, PA, USA.
Bo LinDevelopmental Biology, University of Pittsburgh, Pittsburgh, PA, USA.
Lei YangDevelopmental Biology, University of Pittsburgh, Pittsburgh, PA, USA.
Kimimasa Tobita1] Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA [2] Developmental Biology, University of Pittsburgh, Pittsburgh, PA, USA [3] McGowan Institute of Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.

Funding

Cardiovascular Bioengineering Training ProgramT32HL076124 · NHLBI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI SANJEEV G SHROFF · 2005 to 2026
$7.0M
Three-Dimensional Cardiac Gel Bioreactor for Functioning Cardiomyocyte Induction R21HL094402 · NHLBI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI TOBITA, KIMIMASA · 2009 to 2010
$410k
NHLBI NIH HHS R21 HL094402NHLBI NIH HHS T32 HL076124
6 · The paper itself

Abstract

Cellular cardiomyoplasty has emerged as a novel therapy to restore contractile function of injured failing myocardium. Human multipotent muscle derived stem cells (MDSC) can be a potential abundant, autologous cell source for cardiac repair. However, robust conditions for cardiomyocyte (CM) differentiation are not well established for this cell type. We have developed a new method for CM differentiation from human MDSC that combines 3-dimensional artificial muscle tissue (AMT) culture with temporally controlled biophysical cell aggregation and delivery of 4 soluble factors (microRNA-206 inhibitor, IWR-1, Lithium Chloride, and BMP-4) (4F-AG-AMT). The 4F-AG-AMT displayed cardiac-like response to β-adrenergic stimulation and contractile properties. 4F-AG-AMT expressed major cardiac (NKX2-5, GATA4, TBX5, MEF2C) transcription factors and structural proteins. They also express cardiac gap-junction protein, connexin-43, similar to CMs and synchronized spontaneous calcium transients. These results highlight the importance of temporal control of biophysical and soluble factors for CM differentiation from MDSCs.

Indexed as

Cell DifferentiationBiophysical PhenomenaCell AggregationGene Expression Regulation, DevelopmentalHumansMuscle, SkeletalMyocytes, CardiacStem CellsTranscription FactorsTranscription Factors

Identifiers

PMID25310989
PMCPMC4196107

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.