ArticlePloS one2014
Cytoskeletal dependence of insulin granule movement dynamics in INS-1 beta-cells in response to glucose.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- The Effects of Combined Exercise Training (Resistance-Aerobic) on Serum Kinesin and Physical Function in Type 2 Diabetes Patients with Diabetic Peripheral Neuropathy (Randomized Controlled Trials).Journal of diabetes research · 2020Trial
- ARHGAP18 complexes with both YAP and Merlin and is required for basal actin bundles.Molecular biology of the cell · 2026Article
- The unconventional regulation of non-muscle myosins.Biophysical reviews · 2026Article
- Differential effects of isopropyl alcohol on glucose-induced insulin secretion in INS-1 and MIN6 cells.Pharmacological reports : PR · 2026Article
- Article
- Kinesin-1 autoinhibition tunes cargo transport by motor ensembles.Biophysical journal · 2025Article
- The Rho effector ARHGAP18 coordinates a Hippo pathway feedback loop through YAP and Merlin to regulate the cytoskeleton and epithelial cell polarity.bioRxiv : the preprint server for biology · 2025Article
- Impact of the Microtubule Cytoskeleton on Insulin Transport in Beta Cells: A 3D Computational Study.bioRxiv : the preprint server for biology · 2025Article
- Kinesin-1-transported liposomes prefer to go straight in 3D microtubule intersections by a mechanism shared by other molecular motors.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- In situ structure of actin remodeling during glucose-stimulated insulin secretion using cryo-electron tomography.Nature communications · 2024Article
- Advanced Imaging Techniques for the Characterization of Subcellular Organelle Structure in Pancreatic Islet β Cells.Comprehensive Physiology · 2023Article
- Modulation of insulin secretion by RBFOX2-mediated alternative splicing.Nature communications · 2023Article
- Unveiling nanoscale optical signatures of cytokine-induced β-cell dysfunction.Scientific reports · 2023Article
- Single-molecule analysis of intracellular insulin granule behavior and its application to analyzing cytoskeletal dependence and pathophysiological implications.Frontiers in physiology · 2023Article
- Insulin secretion hot spots in pancreatic β cells as secreting adhesions.Frontiers in cell and developmental biology · 2023Article
- Novel roles of mTORC2 in regulation of insulin secretion by actin filament remodeling.American journal of physiology. Endocrinology and metabolism · 2022Article
- Modeling myosin Va liposome transport through actin filament networks reveals a percolation threshold that modulates transport properties.Molecular biology of the cell · 2022Article
- Organization and dynamics of the cortical complexes controlling insulin secretion in β-cells.Journal of cell science · 2022Article
- Microtubules in Pancreatic β Cells: Convoluted Roadways Toward Precision.Frontiers in cell and developmental biology · 2022Review
- Single-Cell Transcriptomics Links Loss of Human Pancreatic β-Cell Identity to ER Stress.Cells · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
For pancreatic β-cells to secrete insulin in response to elevated blood glucose, insulin granules retained within the subplasmalemmal space must be transported to sites of secretion on the plasma membrane. Using a combination of super-resolution STORM imaging and live cell TIRF microscopy we investigate how the organization and dynamics of the actin and microtubule cytoskeletons in INS-1 β-cells contribute to this process. GFP-labeled insulin granules display 3 different modes of motion (stationary, diffusive-like, and directed). Diffusive-like motion dominates in basal, low glucose conditions. Upon glucose stimulation no gross rearrangement of the actin cytoskeleton is observed but there are increases in the 1) rate of microtubule polymerization; 2) rate of diffusive-like motion; and 3) proportion of granules undergoing microtubule-based directed motion. By pharmacologically perturbing the actin and microtubule cytoskeletons, we determine that microtubule-dependent granule transport occurs within the subplasmalemmal space and that the actin cytoskeleton limits this transport in basal conditions, when insulin secretion needs to be inhibited.
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