ArticleInternational journal of molecular sciences2014
Recombinase polymerase amplification (RPA) of CaMV-35S promoter and nos terminator for rapid detection of genetically modified crops.
Article in International journal of molecular sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 64 citations in OpenAlex.
- Rapid recombinase polymerase amplification-based detection of theGM crops & food · 2026Article
- A multiplex hextuple luciferase assay for advancing cellular signaling analysis in plant cells.Plant communications · 2026Article
- Validation and Improvement of a Rapid, CRISPR-Cas-Free RPA-PCRD Strip Assay for On-Site Genomic Surveillance and Quarantine of Wheat Blast.Journal of fungi (Basel, Switzerland) · 2026Article
- Development of a novel Cas13a/Cas12a-mediated 'one-pot' dual detection assay for genetically modified crops.Journal of advanced research · 2025Article
- A Rapid Field-Visualization Detection Platform for Genetically Modified Soybean 'Zhonghuang 6106' Based on RPA-CRISPR.International journal of molecular sciences · 2024Article
- Application of next-generation sequencing in the detection of transgenic crop.Frontiers in genetics · 2024Review
- Establishment of a recombinase polymerase amplification detection method for Puccinia striiformis f. sp. tritici.Scientific reports · 2023Article
- Development and Visualization Improvement for the Rapid Detection of Decapod Iridescent Virus 1 (DIV1) inViruses · 2022Article
- On point identification of species origin of food animals by recombinase polymerase amplification-lateral flow (RPA-LF) assay targeting mitochondrial gene sequences.Journal of food science and technology · 2021Article
- Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow Immunoassay for Rapid Detection ofFoods (Basel, Switzerland) · 2019Article
- Establishment of a recombinase polymerase amplification (RPA) assay for the detection of Brucella spp. Infection.Molecular and cellular probes · 2019Article
- Article
- Recombinase polymerase amplification: Basics, applications and recent advances.Trends in analytical chemistry : TRAC · 2018Review
- A DNA Barcode-Based RPA Assay (BAR-RPA) for Rapid Identification of the Dry Root of Ficus hirta (Wuzhimaotao).Molecules (Basel, Switzerland) · 2017Article
- Recombinase Polymerase Amplification for Diagnostic Applications.Clinical chemistry · 2016Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recombinase polymerase amplification (RPA) is a novel isothermal DNA amplification and detection technology that enables the amplification of DNA within 30 min at a constant temperature of 37-42 °C by simulating in vivo DNA recombination. In this study, based on the regulatory sequence of the cauliflower mosaic virus 35S (CaMV-35S) promoter and the Agrobacterium tumefaciens nopaline synthase gene (nos) terminator, which are widely incorporated in genetically modified (GM) crops, we designed two sets of RPA primers and established a real-time RPA detection method for GM crop screening and detection. This method could reliably detect as few as 100 copies of the target molecule in a sample within 15-25 min. Furthermore, the real-time RPA detection method was successfully used to amplify and detect DNA from samples of four major GM crops (maize, rice, cotton, and soybean). With this novel amplification method, the test time was significantly shortened and the reaction process was simplified; thus, this method represents an effective approach to the rapid detection of GM crops.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.